黄皮根中欧前胡素的提取及含量测定

Extraction and content determination of imperatorin from Clausena lansinm root

以黄皮(Clausena lansinm)根中欧前胡素含量为考察指标,分别对色谱柱、流动相、检测波长等色谱条件进行探索,采用单因素试验和正交设计法优化供试品溶液的提取条件,测定黄皮根中欧前胡素的含量。结果确定色谱柱为菲罗门Gemini NX C_(18)(5μm,4.6 mm×250 mm),流动相为甲醇-水(55∶45),流速1.0 mL/min,柱温25℃,检测波长300 nm。以超声提取,药材破碎度为过四号筛,提取溶剂为100%甲醇,提取30 min为最佳提取条件。欧前胡素在0.0656~1.6400μg(r=0.9998)线性关系良好,平均加样回收率为97.2%,RSD为0.89%(n=6),该方法可用于黄皮根中欧前胡素的含量测定。

The content rate of imperatorin from Clausena lansinm root was used as the study object,the chromatographic conditions such as chromatographic column,mobile phase and detection wavelength were explored,the preparation conditions of the sample solution were optimized by single factor experiment and orthogonal design,the content of imperatorin from Clausena lansinm root was determined.The chromatographic column was Phenomenex Gemini NXC_(18)(5μm,4.6 mm×250 mm);The mobile phase was methanolwater(55∶45);The flow rate was 1.0 mL/min and the detection wavelength was set at 300 nm with column temperature of 25℃.The extract methods was ultrasonic extraction,the fragmentation degree was acrossing the fourth sieve,extraction solvent was 100%methanol,and extraction time was 30 min.The linear range was 0.0656~1.6400μg(r=0.9998)and the average recovery was 97.2%with RSD of 0.89%(n=6)for imperatorin.The method can be used to determine the content of imperatorin in Clausena lansinm root.