健康人血液中人乳头瘤病毒DNA检测及定量分析

Determination and quantitation of blood human papillomavirus DNA in healthy subjects

目的分析健康献血人群人乳头瘤病毒(HPV)DNA载量,评估其潜在的临床意义。方法采集207名健康献血者的血液,提取基因组DNA,采用GP5+/GP6+引物进行定量聚合酶链反应(PCR),同时对扩增的HPV L1基因140~150 bp的特异性片段进行测序,并进行基因分型。结果207名献血者中,有14名(6.8%)献血者HPV DNA阳性,其中7名为单一型别HPV感染,7名为混合感染。所有阳性样本病毒载量为12.4~39.3拷贝/mL。结论小部分健康人血液中存在低载量的HPV DNA,血液可能是HPV传播的新途径,不排除健康献血者存在潜在的生殖道或其他部位的HPV感染。

Objective To study blood human papillomavirus(HPV)DNA load of healthy subjects,and to evaluate its potential clinical significance.Methods The blood samples from 207 healthy subjects were collected,and genomic DNA was extracted.GP5+/GP6+primers were used for quantitative polymerase chain reaction(PCR).Simultaneously,140-150 bp specific fragment of HPV L1 gene was sequenced to classify genotypes.Results HPV DNA was determined in 14(6.8%)blood donors in the 207 healthy subjects.The 7 cases were single type HPV infection,and 7 cases were mixed infection.The viral load of all the positive samples was 12.4-39.3 copies/mL.Conclusions There is a low load of HPV DNA in the blood of some healthy subjects,and the blood may be a new way of HPV transmission.It is not excluded that some healthy blood donors have potential HPV infection in the reproductive tract or other parts,or have a history of HPV infection.