耐盐脂环胺降解菌的筛选及降解特性研究

Screening of hypersaline tolerant alicyclic aminesdegradation bacterial and its degradation characteristic

通过富集培养从土壤中分离出1株耐盐环己胺降解菌TYUT105,其能以环己胺作为唯一碳源和能源生长,被命名为Bacillus cereus TYUT105。分析该菌对环己胺的降解性能和影响因素,结果表明:该菌在pH为6.0~9.0、温度为20~35℃范围内生长良好;48 h内对10~20 mmol/L的环己胺的去除率达100%;对该菌耐盐性能进行考察,发现该菌在NaCl质量分数为5%的基本盐培养基中生长良好,并可在72 h内完全去除培养基中所含环己胺(10 mmol/L);最后对不同脂环胺内化合物进行了降解研究,发现该菌对其他脂环胺的去除率也可达95%以上。

A hypersaline and cyclohexylamine(CHAM)degrading bacterium TYUT105 was screened from soil,which could grow with the sole carbon source and energy source of CHAM was named as Bacillus cereus TYUT105.The degradation characteristic of TYUT105 on CHAM,was examined.The results showed that the isolated TYUT105 could grow well under pH range from 6.0 to 9.0,temperature from 20℃to 35℃.The removal rate of 10-20 mmol/L CHAM reached 100%within 48 h.The tolerance of TYUT105 toward high concentration of NaCl was tested,and the TYUT105 could degrade 10 mmol/L CHAM under hypersaline conditions(5%NaCl,mass fraction)within 72 h.The degradation of TYUT105 toward other alicyclic amines was also tested,and the removal rate was more than 95%.