豫麦158 及其硬质变异系重要性状基因的KASP 标记检测

Detection of Important Traits Genes with KASP Markers in Yumai 158 and Its Hard Variant Line

豫麦158(审定编号:国审麦2014004)为半冬性小麦品种,籽粒较大,品质较好;豫麦158-42是豫麦158硬质变异系,其籽粒硬度增加,加工品质得到较大改善,达到优质强筋小麦标准。为研究豫麦158-42性状改良的遗传基础,对豫麦158和豫麦158-42的株高、光周期、春化、粒质量、抗逆、品质等相关基因进行KASP(Kompetitive allele specific PCR)标记检测。结果表明,豫麦158-42和豫麦158只有籽粒硬度基因和面粉黄色素含量主效基因不同,其余性状基因均相同。豫麦158同时含有籽粒硬度基因Pinb-D1 a、Pina-D1 a、Pinb2,而豫麦158-42仅含有Pinb2基因,缺失了Pinb-D1 a和Pina-D1 a基因,籽粒硬度较豫麦158增加。豫麦158含有低黄色素含量基因Psy-D1 a,豫麦158-42含有高黄色素含量基因Psy-D1 g。豫麦158和豫麦158-42为小麦籽粒硬度和品质研究提供了重要材料。

Yumai 158(national certification number:2014004)is a semi‑winter wheat cultivar,and it has outstanding performance with relatively large grains and better processing quality.A natural mutation line of Yumai 158 was found in field experiment,and it was designated as Yumai 158‑42.Compared with Yumai 158,Yumai 158‑42 exhibited increased grain hardness and improved processing quality,reaching the standard for high‑quality strong gluten wheat.To investigate the genetic basis of the improved traits of Yumai158‑42,genotyping was performed with KASP(kompetitive allele specific PCR)markers for important genes/loci related to plant height,photoperiod,vernalization,grain weight,stress resistance and quality.The results showed that,Yumai 158 and Yumai 158‑42 had the same genotypes for all the detected genes/loci except for the seed hardness genes and the major‑effect genes related to yellow pigment content.Yumai 158 had the hardness genes Pinb‑D1 a,Pina‑D1 a and Pinb2,while Yumai 158‑42 lost Pinb‑D1 a and Pina‑D1 a,only had Pinb2,and showed increased grain hardness compared with Yumai 158.In addition,Yumai 158 had the low yellow pigment content related gene Psy‑D1 a,while Yumai 158‑42 had the high yellow pigment content related gene Psy‑D1g.Yumai 158 and Yumai 158‑42 are valuable for researches on grain hardness and quality.