摘要
为了解牛肺炎支原体临床分离菌株(HN12)与标准菌株(PG45)蛋白质差异表达情况,采用定量蛋白质组学研究策略,在牛支原体临床分离菌株(HN12)和标准菌株(PG45)的全蛋白质中进行差异蛋白质鉴定,并进行生物信息学分析。结果表明,共鉴定2402个蛋白质,其中存在显著差异的有66个。上调蛋白质数量为25个,下调蛋白质数量为41个。鉴定出的蛋白质主要有膜蛋白、跨膜蛋白、脂蛋白、延长因子、膜脂蛋白P81、伴侣蛋白、可变表面脂蛋白和ABC转运蛋白等。
To understand the differentially expressed proteins‑Bovine Mycoplasma bovis clinical strain(HN12)and standard strain(PG45),this study adopted the quantitative proteomics research strategies,to identify differentially expressed proteins in total proteins of the clinical strain(HN12)and standard strain(PG45)and carried out bioinformatics analysis.The results showed as follows:a total of 2402 proteins were identified,of which 66 were significantly different.Among them,the numbers of up‑regulated and down‑regulated proteins were 25 and 41 respectively.Proteins identified mainly included membrane protein,transmembrane protein,lipoprotein,prolongation factor,membrane lipoprotein P81,chaperone,variable surface lipoprotein,and ABC transporter,et al.
作者
李海利
徐引弟
游一
朱文豪
张青娴
焦文强
王治方
许峰
王克领
郎利敏
张立宪
邹靖
LI Haili;XU Yindi;YOU Yi;ZHU Wenhao;ZHANG Qingxian;JIAO Wenqiang;WANG Zhifang;XU Feng;WANG Keling;LANG Limin;ZHANG Lixian;ZOU Jing(Animal Husbandry and Veterinary Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Xinyang Animal Husbandry Workstation,Xinyang 464000,China)
出处
《河南农业科学》
北大核心
2021年第10期138-145,共8页
Journal of Henan Agricultural Sciences
基金
河南省农业科学院自主创新项目(210221013)。
关键词
牛支原体肺炎
牛支原体
定量蛋白质组学
差异蛋白质
Bovine Mycoplasma pneumonia
Mycoplasma bovis
Quantitative proteomics
Differential protein
