基于香气物质进行乌龙茶核心种质构建与遗传评估

Oolong Tea Core Germplasms Construction and Genetic Evaluation Based on Aroma Metabolites

选择12个代表性的乌龙茶品种(种质)作为供试材料,基于SPME-GC-MS方法获得香气物质表型数据,探索乌龙茶核心种质构建技术流程。当取样比例为25%时,采用多次聚类随机取样法,可以获得‘金牡丹’、‘铁观音’、‘悦茗香’3个核心种质,乙酸苄酯、香叶醇、橙花叔醇、吲哚、芳樟醇及其氧化物等7个核心香气物质。结合SSR标记基因型数据,进行遗传多样性差异分析,核心种质与原生种质2个种群间,在7个表型性状上无显著遗传差异。初步搭建成乌龙茶核心种质构建与遗传评估分析技术流程。

Twelve representative oolong tea cultivars(Camellia sinensis)were used to establish a process for constructing a germplasms classification based on the phenotypic SPME-GC-MS database of the aromatic substances in the plants.At a sampling rate of 25%,Tieguanyin,Yuemingxiang,and Jinmudan were identified as the core germplasms,while linalool oxide I,linalool,caproic acid(Z)-3-hexene ester,aromatic alcohol oxideⅢbenzyl acetate,geraniol,nerolidol,and indolecore the principal aroma metabolites.In addition to the existing SSR marker genotype data,the genetic diversity analysis showed no significant differentiations on 7 phenotypic traits between the core and the primary germplasms.A prototype process based on SSR markers and aromatics in oolong tea plants for constructing a systematic germplasms classification was established.