不同分子质量的人参糖肽复合物增强小鼠免疫功能的研究

Immuno-enhancement of ginseng glycopeptide complex with different molecular weights on mice

研究不同分子质量的人参糖肽复合物对环磷酰胺所致免疫低下小鼠免疫功能的影响。制备人参糖肽总提取物并采用膜分离技术得到重均分子质量为14、11 kDa和<1 kDa的3种人参糖肽,构建BALB/c小鼠注射环磷酰胺致免疫低下动物模型,通过小鼠碳廓清实验、小鼠迟发型变态反应以及外周白细胞总数及自然杀伤细胞(natural killer cell,NK)活性测定实验,对人参糖肽总提取物及不同分子质量的人参糖肽增强小鼠免疫力功能进行评价。结果显示,人参糖肽总提取物及不同分子质量的人参糖肽均具有增强细胞免疫功能、提高单核-巨噬细胞吞噬功能和提升NK细胞活性的作用,<1 kDa的人参糖肽对细胞免疫和增强NK细胞活性有较好的作用,而14 kDa的人参糖肽对改善巨噬细胞吞噬功能有较好的作用。综上所述,人参糖肽复合物以及14、11 kDa和<1 kDa的人参糖肽均具有提高小鼠免疫力的作用,且分子质量较低的人参糖肽复合物作用更明显,可作为开发增强免疫力作用的药品或保健食品的潜在功效成分。

The effect of ginseng glycopeptide complexes with different molecular weight on the immune function of immunocompromised mice induced by cyclophosphamide were studied.The total extract of ginseng glycopeptide was prepared and membrane separation technology was used to obtain three ginseng glycopeptides with weight-average molecular weights of 14,11 kDa and<1 kDa.A BALB/c mouse model of hypoimmunity induced by cyclophosphamide injection was constructed.The mouse carbon clearance experiment,mouse delayed-type allergy,total number of peripheral white blood cells and the activity of natural killers(NK)were used as indicators,the effect of ginseng glycopeptide total extract and different molecular weight ginseng glycopeptides on the immunity of mice was evaluated.The results showed that the total extract of ginseng glycopeptides and ginseng glycopeptides of different molecular weights all enhanced cellular immune function,the phagocytic function of monocytes and macrophages,and the activity of NK cells.Ginseng glycopeptide with a molecular weight of<1 kDa had good effect on cellular immunity and NK activity enhancement,while that of 14 kDa improved the phagocytic function of macrophages.In summary,ginseng glycopeptides complex and ginseng glycopeptides with molecular weights of 14,11 kDa and<1 kDa could improve the immunity of mice,and the ginseng glycopeptides complex with lower molecular weights had more obvious effects,which can be used as potential functional components for developing immune-enhancing drugs or health food.