Claudin-1、E-cad、CyclinD1和nm23在不同分化肺腺癌中的表达及临床意义

Expression and clinical significance of claudin-1,E-cad,CyclinD1 and nm23 in different differentiated lung adenocarcinoma

目的探讨紧密连接蛋白-1(以下简称“Claudin-1”)、E-钙黏素(E-cad)、细胞周期蛋白D1(CyclinD1)和肿瘤转移抑制基因23(nm23)在不同分化肺腺癌肿瘤细胞中的表达情况,探究其与肺腺癌的分化程度及预后的相关性。方法选取2013年3月至2017年3月齐齐哈尔医学院临床病理诊断中心收治的60例肺腺癌手术患者的肺腺癌肿瘤组织标本和肺腺癌癌旁正常组织标本作为研究对象,将肺腺癌肿瘤组织标本设为实验组,将肺腺癌癌旁正常组织标本设为对照组。按照光镜形态学特点将肺腺癌肿瘤组织标本分为高、中、低分化,对标本的癌组织、癌旁与正常组织应用免疫组织进行化学染色,检测Claudin-1、E-cad、CyclinD1和nm23的表达情况。结果实验组的E-cad、nm23的表达低于对照组,差异有统计学意义(P<0.05);实验组的CyclinD1、Claudin-1的高于对照组,差异有统计学意义(P<0.05);高、中分化腺癌中E-cad、nm23的表达高于低分化,差异有统计学意义(P<0.05)。高、中分化腺癌中CyclinD1、Claudin-1的表达低于低分化,差异有统计学意义(P<0.05)。结论Claudin-1、E-cad、nm23、CyclinD1在肿瘤的发生、发展中相互拮抗,从而影响肿瘤的演进,可作为组织学分型、临床分期和预后判断的辅助指标。

Objective To investigate the expression of Claudin-1,E-Cadherin(E-cad),CyclinD1 and non-metastatic 23(nm23)in different differentiated lung adenocarcinoma cells,and to explore their correlation with the degree of differentiation and prognosis of lung adenocarcinoma.Methods From March 2013 to March 2017,the lung adenocarcinoma tumor tissue specimens and normal tissue specimens adjacent to the lung adenocarcinoma of 60 patients undergoing lung adenocarcinoma surgery admitted to the Clinic Pathology Diagnosis Center of Qiqihar Medical College were selected as the research objects.The tumor tissue samples were set as the experimental group,and the normal tissue samples adjacent to the lung adenocarcinoma were set as the control group.According to the morphological characteristics of light microscopy,lung adenocarcinoma tumor tissue samples were divided into high differentiation,medium differentiation and low differentiation.Immunohistochemical staining was used to detect the expressions of Claudin-1,E-CAD,CyclinD1 and nm23 in cancer,adjacent and normal tissues.Samples were respectively obtained from cancer,paracancerous and normal lung tissues.Immunohistochemical staining was implemented to detect the expression of Claudin-1,E-cad,nm-23 and CyclinD1.Results The expressions of E-cad and nm23 in the experimental group were lower than those in the control group,and the differences were statistically significant(P<0.05).The levels of CyclinD1 and Claudin-1 in the experimental group were higher than those in the control group,and the differences were statistically significant(P<0.05).The expression of E-cad and nm23 in highly differentiation was higher than that in low differentiation,and the differences were statistically significant(P<0.05).The expression of CyclinD1 and Claudin-1 in highly differentiation was lower than that in low differentiation,and the differences were statistically significant(P<0.05).Conclusion Claudin-1,E-cad,nm23 and CyclinD1 play an antagonistic role in the occurrence and development of tumors,which affect the evolution of tumors.They can be used as auxiliary indicators for histological classification,clinical stage and prognosis.