植物乳杆菌DMDL 9010降解亚硝酸盐特性及其相关基因挖掘

Characterization of Nitrite Degradation and Mining Related Genes of Lactiplantibacillus plantarum DMDL 9010

笔者前期对植物乳杆菌DMDL 9010(Lactiplantibacillus plantarum DMDL 9010,LP9010)已进行全基因组测序,其NCBI登录号为CP063986-CP063988。该文通过研究LP9010在不同培养条件和培养基成分下的理化指标变化,结合全基因组序列初步分析LP9010降解亚硝酸盐的特性。结果表明,LP9010具有高效降解亚硝酸盐的能力,其亚硝酸盐降解率随着pH的降低而升高;温度为37℃有利于LP9010降解亚硝酸盐;LP9010在营养充分的条件下降解亚硝酸盐的效果最佳,且氧环境和生长因子对其无显著影响(p>0.05)。相应地,在LP9010全基因组中发现了酸耐受(nhaC、gadA、argG和glnR)、冷热耐受(cfa、cspA、hrcA、htpX、cplC、clpE、clpP、clpX和clpL)、氧耐受(catB、trxB、trxA、gor、msrC、msrA和msrB)以及糖转运(pstI、Crr、NagE、ScrA、MtlA、ManX、ManY、ManZ、GatA、GatB、GatC、FruA和FruB)相关的基因,尤其是发现了亚硝酸盐还原酶基因(lmrB和pgl)。总之,LP9010具有高效降解亚硝酸盐的能力和降解亚硝酸盐相关的基因。此结果为植物乳杆菌降解亚硝酸盐的机理提供了新的思路。

Whole-genome sequencing of Lactiplantibacillus plantarum DMDL 9010(LP9010)was first performed,and its corresponding NCBI accession number is CP063986-CP063988.The nitrite degradation performance of LP9010 was preliminarily investigated based on the changes in the physicochemical indicators under different cultivation conditions and medium compositions,which was followed by the whole-genome sequencing analysis.The results revealed that LP9010 can effectively degrade nitrite.The nitrite degradation rate of LP9010 increased with a decrease in the pH.The temperature of 37℃was conducive for nitrite degradation by LP9010.The optimal nitrite degradation performance of LP9010 can be achieved when there is sufficient nutrition,but oxygen and growth factors do not exert significant effects on nitrite degradation(p>0.05).Furthermore,acid tolerance-related genes(nhaC,gadA,argG,and glnR),cold-and heat-tolerance-related genes(cfa,cspA,hrcA,htpX,cplC,clpE,clpP,clpX,and clpL),oxygen tolerance-related genes(catB,trxB,trxA,gor,msrC,msrA,and msrB),and sugar transport-related genes(pstI,Crr,NagE,ScrA,MtlA,ManX,ManY,ManZ,GatA,GatB,GatC,FruA,and FruB)were found in the whole genome sequencing of LP9010.Besides,the nitrite reductase genes(lmrB and pgl)were discovered.Conclusively,LP9010 can degrade nitrite efficiently and it possesses the genes related to nitrite degradation.This article provides a new direction to better understand and elucidate the nitrite degradation mechanism of Lactiplantibacillus plantarum.