TLR9和NF-κB在尖锐湿疣组织中的表达

Expression of TLR9 and NF-κB in condyloma acuminatum

目的探讨尖锐湿疣(CA)组织中Toll样受体9(TLR9)和核因子κB(NF-κB)的表达及意义。方法采用免疫组化SP法检测TLR9和NF-κB在50例CA组织和20例正常包皮组织中的表达情况,并进行比较。采用Spearman分析检测CA组织中TLR9和NF-κB表达的相关性。结果50例CA患者组织中,TLR9和NF-κB的阳性表达率分别为94.00%(47/50)和98.00%(49/50),表达强度多为++~+++。20例正常包皮组织中,TLR9和NF-κB的阳性表达率分别为10.00%(2/20)和20.00%(4/20),表达强度多为-~+。两组TLR9和NF-κB的阳性表达率和阳性表达强度差异均有统计学意义(均P<0.05)。CA组织中TLR9和NF-κB的表达呈正相关(r=0.57,P<0.05)。结论CA组织中存在TLR9和NF-κB的过表达,可能与人乳头瘤病毒感染后被过度激活有关。二者可能对CA的发生、发展有一定作用。

Objective To explore the expression and significance of TLR9 and NF-κB in condyloma acuminatum(CA).Methods The streptavidin-peroxidase(SP)immunohistochemistry method was used to measure the expression of TLR9 and NF-κB in 50 cases of CA and 20 normal controls.Results The positive expression rates of TLR9 and NF-κB were 94.00%(47/50)and 98.00%(49/50)in CA tissues,and 10.00%(2/20)and 20.00%(4/20)in the normal controls.The staining intensities of TLR9 and NF-κB in CA were significantly higher(++~+++)than those in normal controls(-~+).Positive expression rates and staining intensities of TLR9 and NF-κB were significantly different between CA and normal controls(P<0.05).Moreover,the positive rates TLR9 and NF-κB staining in CA were positively correlated(r=0.57,P<0.05).Conclusions TLR9 and NF-κB are overexpressed in CA tissues,which is likely associated with the hyperactivation after HPV infection.TLR9 and NF-κB could be involved in the development and the progress of CA.