返魂草中肝毒性吡咯里西啶生物碱的含量分析及风险评估

Risk Assessment and Determination of Hepatotoxic Pyrrolizidine Alkaloids in Senecionis Cannabifolii Herba

目的对返魂草中肝毒性吡咯里西啶生物碱的含量进行测定,并根据测定结果进行初步的风险评估。方法建立超高效液相色谱-串联质谱测定夹可灵、夹可灵氮氧化物、夹可碱、夹克宁和夹可碱氮氧化物的含量。根据含量测定结果,采用暴露限值(margin of exposure,MOE)法进行风险评估。结果6批返魂草样品中夹可灵、夹可灵氮氧化物、夹可碱、夹克宁和夹可碱氮氧化物的含量范围分别为0.23~0.85、0.25~0.98、0.85~2.65、0.37~1.85和2.32~7.58μg/g。MOE值为2131~4845。结论本研究所建立的方法为返魂草中肝毒性吡咯里西啶生物碱的质量控制和安全性评价提供了科学依据。

Objective To analyze the contents of hepatotoxic pyrrolizidine alkaloids in Senecionis cannabifolii Herba,and to carry out preliminary risk assessment based on the research results.Methods A UPLC-MS/MS method was established to determine the contents of jacoline,jacoline N-oxide,jacobine,jaconine and jacobine N-oxide.According to the content determination results,the risk was assessed using the margin of exposure(MOE)method.Results The contents of jacoline,jacoline N-oxide,jacobine,jaconine,and jacobine N-oxide in six batches of Senecionis cannabifolii Herba were 0.23-0.85,0.25-0.98,0.85-2.65,0.37-1.85 and 2.32-7.58μg/g,respectively.The MOE values ranged from 2131 to 4845.Conclusion The method established in this study provides data for quality control and safety evaluation of hepatotoxic pyrrolizidine alkaloids in Senecionis cannabifolii Herba.