SM22α在上皮性卵巢癌组织中的表达及意义

Expression and significance of SM22αin the tissues of epithelial ovarian cancer

目的检测平滑肌22α蛋白(SM22α)在上皮性卵巢癌组织中的表达,探讨其在卵巢癌发生发展中的作用及意义。方法选取2015年12月至2018年12月石家庄市第一医院妇科手术治疗的上皮性卵巢癌患者卵巢癌组织标本43例为试验组,选取同期因宫颈癌或子宫肌瘤行全子宫双附件切除患者的正常卵巢组织50例为对照组。荧光实时定量PCR(qRT-PCR)技术检测2组标本SM22α基因mRNA的表达情况,Western blot检测2组标本SM22α蛋白表达情况,并分析SM22α基因mRNA及蛋白表达与卵巢癌患者临床病理特征之间的关系。结果卵巢癌组织中SM22αmRNA及其蛋白表达水平显著低于正常卵巢组织(P<0.05),且卵巢癌组织中SM22αmRNA表达水平与其蛋白表达呈正相关性(r=0.701,P<0.05)。此外,卵巢癌组织中SM22αmRNA及其蛋白表达水平表达与卵巢癌患者的淋巴结转移有相关性(P<0.05)。结论SM22α表达下调可能在卵巢癌发生、发展过程中发挥重要作用,其有可能成为卵巢癌分子诊断和治疗的重要靶点。

Objective To investigate the expression and significance of SM22αin the tissues of epithelial ovarian cancer,and to explore its role and significance in the pathogenesis and development of ovarian cancer.Methods A total of 43 ovarian cancer tissue specimens collected from 43 patients with ovarian cancer who were treated in our hospital from December 2015 to December 2018 were enrolled as experimental group,and 50 normal ovarian tissues specimens collected from 50 patients with cervical cancer or uterine leiomyoma who underwent total hysterectomy in our hospital at the same period were enrolled as control group.The expression levels of SM22αmRNA and protein were detected by qRT-PCR and Western Blot,respectively.Moreover the correlation between the expression levels of SM22αand clinicopathological characteristics was analyzed.Results The expression levels of SM22αmRNA and protein in ovarian cancer tissues were significantly lower than those in normal ovarian tissues(P<0.05).Moreover the expression levels of SM22αmRNA in ovarian cancer tissues were positivelt correlated with those of SM22αprotein(r=0.701,P<0.05).In addition,there was a significant correlation between the expression levels of SM22αmRNA and protein in ovarian cancer tissues and lymph node metastasis of patients(P<0.05).Conclusion The downregulation of SM22αexpression may play an important role in the pathogenesis and development of ovarian cancer,which may become an important target for molecular diagnosis and treatment of ovarian cancer.