超量表达DWF4基因对芥菜生长发育的影响

Effect of DWF4 Gene Overexpression on Growth and Development in Brassica juncea

为研究DWF4基因在芥菜生长过程中的作用与功能,也为今后开发生物技术手段调控植物生长发育奠定理论基础,通过农杆菌介导转化法将DWF4基因导入芥菜中,结果显示:转基因芥菜植株生长迅速,生长势旺盛,其叶、花等器官都大于对照野生型.转基因植株成长过程中,其株高、开展度、最大叶宽、最大叶长都显著高于野生型,且植株抽薹时间和开花时间提前.pCA BarDWF4转基因芥菜植株与野生型植株相比,主枝更长,分枝更多,总种荚数增多,总种子数增多,种荚率更高.对pCA BarDWF4转基因芥菜植株定量PCR分析结果显示:植株体内油菜素甾醇(Brassinosteroids,BR)合成途径相关基因表达增高.结果表明:DWF4基因能促进植株内源BR的生成,因此促进了植株的生长发育.

In order to understand the role and function of DWF4 gene in the growth process of mustard(Brassica juncea Coss.)and to lay a theoretical foundation for the development of biotechnology to regulate plant growth and development in the future,DWF4 gene was introduced into mustard by means of Agrobacterium-mediated transformation.Compared with the control,i.e.the wild type,the plants of pCABarDWF4 transgenic mustard grewmorerapidly and vigorously,with larger leaf,flower and other organs,had significantly greater plant height,plant expansion,maximum leaf width and maximum leaf length and longer podding branches,more branches,more pods,higher seed rate and more seeds,and their bolting time and flowering time were advanced.Quantitative PCR analysis of pCA BarDWF4 transgenic mustard plants showed that DWF4 gene enhanced the expression of the genes related to endogenous BR synthesis pathways in the plant.In conclusion,the DWF4 gene promotes the endogenous BR synthesis in mustard and thus favors the growth and development of the plant.