甜荞FePG1基因克隆及表达分析

Gene Cloning and Expression Analysis of FePG1 in Common Buckwheat(Fagopyrum esculentum)

基于前期转录组测序结果,从自交不亲和甜荞乌克兰大粒荞材料中克隆了一个多聚半乳糖醛酸酶(Polygalacturonase PG)编码基因,命名为FePG1;该基因CDS全长1215 bp,编码404个氨基酸,含有一个PL-6超家族保守结构域,包含一个信号肽,为稳定的疏水性跨膜蛋白.通过亚细胞定位检测发现FePG1基因主要在细胞膜和细胞质中表达,推测FePG1蛋白为分泌蛋白并可能在甜荞花柱的形态形成上有重要功能.qRT-PCR试验结果表明,FePG1基因在甜荞盛花期的花中高表达,且在短柱花雌雄蕊中高表达,长柱花雌雄蕊中低表达,推测甜荞FePG1基因可能与甜荞花柱异长发育有关.

A polygalacturonase gene,named as FePG1,has been cloned in the self-incompatible common buckwheat variety‘Ukrainian Daliqiao',based on our former transcriptome analysis result.FePG1,CDS full length 1215 bp,encodes 404 amino acids,contains a signal peptide,which is a stable hypdrophobicity protein and belongs to PL-6 superfamily protein domain.According to subcellular localization,FePG1 is mainly expressed in cytomembrane and cytoplasm.It is speculated that FePG1 protein is a secretory protein and may play an important role in the style formation of common buckwheat.qRT-PCR showed that FePG1 has high expression in flower of common buckwheat at the full-bloom stage.Especially,it has higher expression in S-morph Flower than L-morph Flower,which speculates that FePG1 might be related to the style development of the self-incompatible common buckwheat.