响应面分析法优化黑松松花粉多糖提取工艺

Optimization of extraction process of black pine pollen polysaccharide by response surface analysis

以黑松松花粉为原料,采用超声波和纤维素酶辅助提取松花粉多糖。在单因素试验的基础上采用响应面分析方法,得到提取工艺的最优条件。结果表明:影响松花粉多糖得率的因素按主次顺序分别为水浴时间>酶解p H>纤维素酶添加量>水浴温度,多糖提取的最佳工艺条件为纤维素酶添加量1.5%(以松花粉质量为基准)、水浴温度51℃、酶解p H 6.0、水浴时间50 min,在此条件下松花粉多糖得率预测值为2.08%,验证值为2.00%。

The extraction of pine pollen polysaccharides was performed with the aid of ultrasonic and cellulase using black pine pollen as raw material.On the basis of single factor experiment,response surface method(RSM)was used to optimized the conditions of extraction process.The results showed that the factors influencing the polysaccharide yield of pine pollen were water bath time>enzymatic p H>cellulase addition>water bath temperature,and the optimal conditions for polysaccharide extraction were cellulase addition 1.5%(based on the mass of pine pollen),water bath temperature 51℃,enzymatic p H 6.0,water bath time 50 min,under these conditions,the predicted value of pine pollen polysaccharide yield was 2.08%and the validated value was 2.00%.