细胞因子基因多态性及Hp感染与慢性萎缩性胃炎易感性的关系

Relationship between cytokine gene polymorphism,Hp infection and susceptibility to chronic atrophic gastritis

目的探讨细胞因子白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)基因多态性及幽门螺杆菌(Hp)感染与慢性萎缩性胃炎(CAG)易感性的关系。方法选取2018年1月-2020年7月萍乡矿业集团有限责任公司总医院收治的CAG患者82例为研究对象进行横断面研究,同时选取医院同期体检的80名健康人作为对照组,采用^(14)C-UBT检测两组Hp感染情况,同时分别采集两组样本空腹肘静脉血5ml于EDTA抗凝管中,提取DNA并采用聚合酶链式反应(PCR)定量扩增技术检测IL-6和TNF-α基因多态性,然后分析其对CAG易感性的影响。结果CAG组Hp阳性率高于对照组(P<0.05);CAG组和对照组IL-6572C/G、TNF-α308A/G和TNF-α1031T/C位点基因型和基因频率分布差异均有统计学意义(P<0.05);分别以IL-6572GG、TNF-α308GG、TNF-α863CC和TNF-α1031TT基因型为对照,根据Hp感染情况进行分层研究显示Hp阳性组IL-6572CC、TNF-α308GA和TNF-α1031CT基因型携带者CAG发病的OR值降低(P<0.05),Hp阴性组TNF-α1031CT基因型OR值降低(P<0.05),非条件Logistic回归分析显示IL-6572C/G和TNF-α308A/G位点基因多态性与Hp感染对CAG发病具有交互作用(P<0.05),TNF-α1031T/C位点基因多态性与Hp感染无交互作用。结论IL-6572C/G、TNF-α308A/G和TNF-α1031T/C基因多态性以及Hp感染均可导致CAG疾病易感性,且IL-6572C/G和TNF-α308A/G基因多态性与Hp感染存在交互作用。

OBJECTIVE To investigate the relationship between gene polymorphisms of cytokines of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α),Helicobacter pylori(Hp)infection and susceptibility to chronic atrophic gastritis(CAG).METHODS 82patients with CAG admitted to department of gastroenterology of General Hospital of Pingxiang Mining Group Co.,Ltd.between Jan.2018and Jul.2020were selected as study subjects for a cross-sectional study,and 80healthy people who received physical examination in the hospital during the same period were selected as control group.^(14)C-UBT was used to detect Hp infection in the two groups.At the same time,5mL of fasting cubital venous blood were collected from two groups in EDTA anticoagulation tube,DNA was extracted and polymerase chain reaction(PCR)quantitative amplification technique was used to detect the gene polymorphisms of IL-6and TNF-α,and their effects on CAG susceptibility were analyzed.RESULTS The positive rate of Hp in CAG group was significantly higher than that in control group(P<0.05).There were significant differences in the genotypes and gene frequency distribution at IL-6572C/G,TNF-α308A/G and TNF-α1031T/C loci between CAG group and control group(P<0.05).Taking TT genotypes at IL-6572GG,TNF-α308GG,TNF-α863CC and TNF-α1031locus as controls,a stratified study based on Hp infection showed that the OR values of CAG onset in patients with genotypes of IL-6572CC,TNF-α308GA and TNF-α1031CT were significantly reduced in Hp-positive group(P<0.05),and the OR value of TNF-α1031CT genetype was significantly reduced in Hp-negative group(P<0.05).Unconditional Logistic regression analysis showed that gene polymorphisms of IL-6572C/G and TNF-α308A/G loci and Hp infection had an interactive effect on the pathogenesis of CAG(P<0.05),while the gene polymorphism of TNF-α1031T/C locus had no interaction with Hp infection.CONCLUSIONThe gene polymorphisms of IL-6572C/G,TNF-α308A/G and TNF-α1031T/C,and Hp infection could all lead to CAG susceptibility,and gene polymorphisms IL-6572C/G and TNF-α308A/G interacted with Hp infection.