基于生物信息学分析骨质疏松症与阿尔茨海默症的相互关系

Analysis of the relationship between osteoporosis and Alzheimer’s disease based on bioinformatics

目的利用生物信息学分析骨质疏松症与阿尔茨海默症差异表达的miRNA及两病的相互关系。方法利用GEO数据库获取骨质疏松症(osteoporosis,OP)与阿尔茨海默症(Alzheimer’s disease,AD)的基因芯片,使用GEO2R在线分析得到OP与AD差异表达的微小核糖核酸(differentially expressed microRNA,DEmiRNA),将筛选后的DEmiRNAs用miRDB数据库和Targetscan数据库进行靶基因预测;用DAVID数据库对靶基因进行GO分析和KEGG信号通路分析,并结合STRING数据库,制作蛋白互作网络和DEmiRNAs-mRNA调控网络。结果OP与AD的基因芯片中共有9个有意义的DEmiRNAs,通过蛋白互作网络筛选出前10个核心基因为:COL1A1、VEGFA、COL4A1、COL3A1、COL5A1、COL2A1、LOX、COL7A1、IGF1、COL5A2,同时发现hsa-miR-29b-3p和胶原蛋白在两病中的关系最为密切。结论通过研究两种疾病DEmiRNAs与核心基因有助于了解OP与AD的相互关系,了解两病共同的发病机理,为两病治疗提供新的思路与方向。

Objective To analysis the differentially expressed miRNAs and the relationship between osteoporosis and Alzheimer’s disease based on bioinformatics.Methods The gene chips of osteoporosis(OP)and Alzheimer’s disease(AD)were obtained using Gene Expression Omnibus(GEO)database.The differentially expressed microRNAs(DEmiRNAs)between OP and AD were obtained using GEO2R online analysis.Target genes for the selected DEmiRNAs were predicted using miRDB database and Targetscan database;The DAVID database was used to analyze the target genes with GO analysis and KEGG signal pathway analysis.A protein interaction network and a DEmiRNAs-mRNA regulatory network were created with the STRING database.Results There were 9 meaningful DEmiRNAs in the gene chips of OP and AD.The top 10 core genes,COL1A1,VEGFA,COL4A1,COL3A1,COL5A1,COL2A1,LOX,COL7A1,IGF1,and COL5A2,were screened through the protein interaction network.At the same time,it was found that hsa-miR-29b-3p and collagen were most closely related to OP and AD.Conclusion By studying the DEmiRNAs and core genes of the two diseases,it is helpful to understand the relationship between OP and AD,to understand the common pathogenesis of the two diseases,and to provide new ideas and directions for the treatment of the two diseases.