转录因子PU.1和KLF7在小鼠组织中的基因表达分析

Expression Analysis of Transcription Factors PU.1 and KLF7 in Mouse Tissue

转录因子PU.1和KLF7在脂肪形成、造血分化、肿瘤发生和机体免疫等方面发挥重要作用,但是两者在功能上是否具有相关性尚不清楚。本研究旨在研究PU.1和KLF7在小鼠不同组织中的表达模式及相关性。利用RT-qPCR检测PU.1和KLF7在小鼠胃、肝脏、空肠、肺、腿肌、心脏、脂肪、胸肌、脾脏、肾脏和脑组织中的表达情况,并分析表达相关性。结果显示,KLF7在肺、胃和脂肪中的表达量较高,PU.1在肺、肝脏和脾脏中的表达量较高;两个基因的表达呈极显著正相关(r=0.857, P=0.001)。生物信息学预测显示,在KLF7的5’侧翼区共存在5个PU.1的结合位点,PU.1可能正调控KLF7基因的表达。本研究为深入研究PU.1和KLF7的功能及相互作用机制提供重要的基础数据。

Transcription factors PU.1 and KLF7 play important roles in adipogenesis, hematopoiesis, tumorigenesis, and body immune, but whether they are functionally related is unclear. The purpose of this study was to investigate the expression pattern and correlation of PU.1 and KLF7 in different tissue of mices. The expressions of PU.1 and KLF7 in the stomach, liver, jejunum, lung, leg muscle, heart, fat, chest muscle, spleen, kidney and brain tissues of mice were detected by RT-qPCR, and the correlation was analyzed. The results showed that KLF7 was highly expressed in lung, stomach and fat, and PU.1 was highly expressed in lung, liver and spleen. The expression of the two genes was significantly positively correlated(r=0.857, P=0.001). Bioinformatics prediction showed that there were 5 binding sites of PU.1 located in the 5’-flanking region of KLF7, which may regulate the expression of KLF7 gene. This study provided important basic data for in-depth study of the function and interaction mechanism of PU.1 and KLF7.