哒螨灵和高温胁迫下截形叶螨的应激反应

Stress response of Tetranychus truncatus to pyridaben and high temperatures

【目的】测定截形叶螨Tetranychus truncatus敏感品系(SS)、抗哒螨灵品系(Py-R)体内的自由基和保护酶的变化,探究截形叶螨在药剂和高温的双重胁迫下的应激反应,为叶螨的综合治理提供一定的理论基础。【方法】经哒螨灵和38℃高温处理后,测定截形叶螨不同品系体内的可溶性蛋白含量、超氧自由基(·O_(2)^(-))和超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,并分析自由基和3种保护酶的变化与药剂和高温双重胁迫的相关性。【结果】截形叶螨敏感品系和抗性品系经哒螨灵和高温处理后体内·O_(2)^(-)含量分别是对照的5.5192倍和1.3462倍,SOD活性分别是对照的5.3447倍和7.1052倍,CAT和POD活性也均高于对照。【结论】截形叶螨体内的·O_(2)^(-)、SOD、CAT和POD与哒螨灵药物和高温胁迫有关,Py-R在长期哒螨灵胁迫作用下对保护酶活性的诱导能力加强。

[Objectives]To investigate changes in free radical and protective enzyme levels induced by exposure to pyridaben and a high temperature,in a sensitive(SS)and pyryl-R resistant(Py-R)strain of Tetranychus truncatus.[Methods]Soluble protein content and activity of the superoxide radical(·O_(2)^(-)),superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),in the SS and Py-R strains were determined after treatment with pyridaben and exposure to 38℃.The interaction between free radicals and three protective enzymes was also analyzed.[Results]The·O_(2)^(-) content of the SS and Py-R strains were 5.5192 and 1.3462 times higher than that of the control group after exposure to pyridaben and the high temperature,and SOD activity was 5.3447 and 7.1052 times higher.CAT and POD activity were also higher than in the control group.[Conclusion]·O_(2)^(-),SOD,CAT and POD levels in T.truncatus are affected by pyridaben and high temperature stress,and the Py-R strain has higher protective enzyme activity under long-term pyridaben stress.