miR-206在血管瘤患儿外周血清中的表达及其与普萘洛尔疗效的相关性

Expression of miR-206 in peripheral blood of hemangioma children and its correlation with the efficacy of propranolol

目的探讨微小RNA-206(micro RNA-206,miR-206)在血管瘤患儿外周血中的表达及其与普萘洛尔疗效的相关性。方法检测45例接受普萘洛尔治疗的血管瘤患儿外周血清中miR-206表达水平,将患儿按照miR-206均值分为高表达组(n=19)和低表达组(n=26),比较两组临床特征;将患儿根据治疗6个月后的疗效分为疗效良好组(n=29)和疗效一般组(n=16),采用Logistic回归分析miR-206是否是疗效的影响因素,采用ROC曲线评价miR-206对普萘洛尔治疗血管瘤疗效的预测价值。结果高表达组在<6月龄、增生期、瘤体面积≥10 cm2的患儿比例显著低于低表达组(分别χ^(2)=4.664、7.207、8.927,P=0.031、0.007、0.012);治疗后患儿miR-206表达水平(3.25±0.64)显著高于治疗前(2.12±0.41)水平(t=9.973,P<0.05);普萘洛尔治疗6个月后,疗效良好组患儿治疗前miR-206高表达比例(24.1%)显著低于疗效一般组(75.0%)(χ^(2)=10.934,P=0.001);治疗前miR-206高表达是普萘洛尔治疗血管瘤患儿疗效不良的危险因素[OR(95%CI)=6.423(1.436~28.731),P=0.015];ROC曲线分析显示,血清miR-206预测普萘洛尔治疗血管瘤疗效的ROC曲线下面积为0.798,敏感性为0.83,特异性为0.75。结论血清miR-206表达与血管瘤患儿年龄、是否增生期及瘤体面积有关,这可能是预测婴幼儿血管瘤普萘洛尔疗效的重要指标之一。

Objective To investigate the expression of microRNA(miR)-206 in the peripheral serum of hemangioma children and its correlation with curative effect of propranolol treatment.Methods The expression of miR-206 in serum of 45 hemangiomas children treated with propranolol treatment was detected.The children were divided into the high expression group(n=19)and the low expression group(n=26)according to the mean value of miR-206,and the clinical characteristics of the two groups were compared;The children were divided into the good efficacy group(n=29)and general efficacy group(n=16)according to the efficacy after 6 months of treatment.Logistic regression was used to analyze whether miR-206 was an influencing factor of efficacy,and ROC curve was used to evaluate the predictive value of miR-206.Results The proportion of children<6 months,in hyperplasia stage and with tumor area≥10 cm2 in the high expression group was significantly lower than that in the low expression group(χ^(2)=4.664,7.207,8.927,P=0.031,0.007,0.012,respectively).The miR-206 level after treatment(3.25±0.64)was significantly higher than that before treatment(2.12±0.41,t=9.973,P<0.05).After six months of propranolol treatment,the proportion of high expression of miR-206 before treatment in the good efficacy group(24.1%)was significantly lower than that in the general efficacy group(75.0%,χ^(2)=10.934,P=0.001).High expression of miR-206 before treatment was a risk factor for poor curative effect of propranolol in hemangioma children[CI(95%CI)=6.423(1.436~28.731),P=0.015];ROC curve analysis showed that the area under ROC curve of miR-206 for predicting the efficacy of propranolol was 0.798,the sensitivity was 0.83,and the specificity was 0.75.Conclusion The expression of serum miR-206 was correlated with age,proliferative stage and tumor area and may be one of the important indicators for predicting propranolol effect in children with hemangioma.