UFLC-MS/MS法同时测定宁泌泰胶囊中没食子酸、连翘苷、盐酸小檗碱、盐酸巴马汀和槲皮素在大鼠血浆中的含量

Simultaneous determination contents of gallic acid, forsythin, berberine hydrochloride, palmatine hydrochloride and quercetin of Ningmitai Capsules in plasma of rats by UFLC-MS/MS method

目的建立同时测定大鼠ig宁泌泰胶囊后血浆中没食子酸、连翘苷、盐酸小檗碱、盐酸巴马汀和槲皮素5种成分的超快速液相色谱串联质谱(UFLC-MS/MS)方法。方法大鼠ig宁泌泰胶囊(1.5、4.0g/kg)或iv没食子酸、连翘苷、盐酸小檗碱、盐酸巴马汀和槲皮素5种成分的混合溶液,采集不同时间点的血样。采用甲醇沉淀血浆蛋白,以乙酰对氨基酚为内标,进行UFLC-MS/MS分析。结果没食子酸、连翘苷、盐酸小檗碱、盐酸巴马汀和槲皮素5种成分在大鼠血浆中呈良好的线性关系。方法学考察均符合要求,日内、日间精密度RSD≤15%,稳定性良好,准确度为90.60%-113.50%,提取回收率为73.77%-94.86%,基质效应为83.80%-106.17%。结论建立的UFLC-MS/MS方法可以快速、灵敏地检测生物样品中没食子酸、连翘苷、盐酸小檗碱、盐酸巴马汀和槲皮素的血药浓度,可以用于宁泌泰胶囊中5种成分的药动学研究。

Objective To establish an ultra-fast liquid chromatography tandem mass spectrometry(UFLC-MS/MS) method for the simultaneous determination of five components of gallic acid, forsythin, berberine hydrochloride, palmatine hydrochloride and quercetin in the plasma of rat after ig Ningmitai Capsules(宁泌泰胶囊). Methods Rats were ig Ningmitai Capsules(1.5, 4.0 g/kg)or iv gallic acid, forsythin, berberine hydrochloride, palmatine hydrochloride and quercetin mixed solution, blood sample were collected at different time points. The plasma protein was precipitated with methanol, and acetaminophen was used as internal standard for UFLC-MS/MS analysis. Results Gallic acid, forsythin, berberine hydrochloride, palmatine hydrochloride and quercetin showed a good linear relationship in plasma of rats. The methodological investigations met the requirements. RSD of intraand inter-day precision were less than 15%, the stability was good, the accuracy was 90.60%—113.50%, the absolute recovery was 73.77%—94.86%, and the matrix effect was 83.80%—106.17%. Conclusion The established UFLC-MS/MS method can quickly and sensitively detect the plasma concentrations of gallic acid, forsythin, berberine hydrochloride, palmatine hydrochloride and quercetin in biological samples, and can be used for the pharmacokinetic study of five components in Ningmitai Capsules.