丽水地区甲型流感病毒H1N1感染患者上呼吸道微生态分析

Microecological analysis of upper respiratory tract in patients with influenza A virus H1N1 infection in Lishui region

目的通过微生物16S r DNA测序技术,分析丽水市人民医院发热门诊收治的126例H1N1感染患者的咽拭子标本。方法 2018年12月-2019年3月丽水市人民医院发热门诊收治的126例作为H1N1感染组,对照组为自愿加入研究长期定居在丽水的健康者,共100例。提取细菌基因组DNA,利用紫外分光光度计Nenodrop定量后进行16S r DNA测序分析。结果健康对照组和H1N1感染组的菌群丰度前10位的菌群组成基本相同,分别是芽胞杆菌纲、γ-变形菌纲、放线菌纲、β-变形菌纲、拟杆菌纲、Negativicutes、梭杆菌纲、梭菌纲、黄杆菌纲和α-变形菌纲。其中丰度在20%以上的为芽胞杆菌纲,10%~20%的为γ-变形菌纲和放线菌纲,在5%~10%的为β-变形菌纲、拟杆菌纲及Negativicutes,低于5%且丰都在前10位分别为梭杆菌纲、梭菌纲、黄杆菌纲和α-变形菌纲。与健康对照组相比,甲型H1N1病毒感染患者上呼吸道菌群中放线菌纲和黄杆菌纲明显降低。结论 H1N1感染会引起上呼吸道定植菌群的变化,根据这些变化可以为后续抗生素的支持治疗提供选择依据。

Objective The pharyngeal swabs of 126 patients with H1 N1 infection admitted to Fever Clinic of Lishui People’s Hospital were analyzed by microbial 16 S r DNA sequencing technology. Methods A total of 126 patients admitted to the Fever Clinic of Lishui People’s Hospital from December 2018 to March 2019 were included in the H1 N1 infection group,and 100 healthy people who settled in Lishui for a long time and voluntarily enrolled in the study were collected into the control group.Bacterial genomic DNA was extracted,and Nenodrop-quantification was performed by ultraviolet spectrophotometer for 16 S r DNA sequencing analysis. Results The composition of the top ten bacteria in the abundance of the control group and the H1 N1 infected group was basically the same,which were Bacillus,γ-proteobacteria,Actinomycetes,β-proteobacteria,Bacteroidetes,Negativicutes,Fusobacterium,Clostridium,Flavobacteria and α-proteobacteria,respectively. Among them,the abundance of more than 20% are Bacillus;10%-20% are γ-proteobacteria and Actinomycetes,5%-10% are β-proteobacteria,Bacteroidetes and Negativicutes;less than 5% and in the top ten are Fusobacterium,Clostridium,Flavobacteria and α-proteobacteria,respectively. Actinobacteria and flavobacteria were significantly reduced in the upper respiratory tract of patients infected with A( H1 N1) virus compared with the controls. Conclusion H1 N1 infection can cause changes in upper respiratory colonization flora,and these changes can provide basis for subsequent antibiotic supportive treatment.