白细胞介素33在结直肠癌患者癌组织中的表达及其与复发的相关性研究

The expression of interleukin-33 in carcinoma tissues and its relationship with recurrence in patients with colorectal cancer

目的探讨结直肠癌(colorectal cancer,CRC)患者癌组织中白细胞介素33(IL-33)表达水平与复发的相关性。方法纳入2014年6月1日-2020年6月30日于宁波鄞州肛肠医院和新乡医学院第一附属医院就诊的164例CRC患者和80例腹部外伤行剖腹探查的非结直肠癌患者作为研究对象,采用免疫组化方法检测并比较2组结直肠组织中IL-33表达水平的差异。另纳入3例CRC患者和3例腹部外伤行剖腹探查的非结直肠癌患者作为独立验证样本,采用Western bolt法检测癌组织、癌旁组织及正常结直肠组织中IL-33表达水平的差异,结直肠癌患者术后复发的影响因素采用Cox风险比例模型分析。结果 CRC患者癌组织、癌旁组织及正常结直肠组织中IL-33蛋白表达水平差异有统计学意义(F=13.726,P=0.001),与正常结直肠组织和CRC患者癌旁组织相比,CRC患者癌组织中IL-33表达水平显著升高,差异均有统计学意义(P<0.05);与癌旁组织相比,CRC患者癌组织中IL-33蛋白表达水平显著升高,差异有统计学意义(P<0.05)。IL-33高表达组和低表达组复发率分别为57.02%和25.58%,高表达组复发率显著升高(χ^(2)=12.554,P=0.000)。淋巴结转移、TNM分期和IL-33是影响CRC患者复发的独立危险因素。结论结直肠癌患者癌组织中IL-33表达水平显著升高,IL-33与结直肠癌患者不良预后有关。

Objective To investigate the expression of interleukin-33( IL-33) in carcinoma tissues and its relationship with recurrence in patients with colorectal cancer( CRC). Methods A total of 164 patients with CRC and 80 patients with abdominal trauma who underwent exploratory laparotomy between June 1,2014 and June 31,2020 in Yinzhou Anorectal Hospital and The First Affiliated Hospital of Xinxiang Medical University were enrolled. The expression of IL-33 in the colorectal tissue of all individuals were detected and compared by immunohistochemical methods. Another 3 CRC patients and 3 abdominal trauma who underwent exploratory laparotomy were also enrolled as independent verification samples,and the difference of IL-33 expression in colorectal tissue of the two groups was detected by Western bolt method. The influencing factors of postoperative recurrence in CRC patients were analyzed by Cox regression model. Results There was statistical significance on the differences in the expression of IL-33 protein in the cancerous,para-carcinoma tissues and normal colorectal tissue( F = 13. 726,P = 0. 001). Compared with normal colorectal tissue and para-carcinoma tissue,IL-33 protein expression in cancerous tissues of CRC patients was significantly increased,and the difference was statistically significant( P < 0. 05). IL-33 protein expression level in cancerous tissues of CRC patients was significantly higher when compared with para-carcinoma tissues,and the difference was statistically significant( P < 0. 05). The recurrence rate of the IL-33 high expression group and the low expression group were 57. 02% and 25. 58%,respectively,and the recurrence rate of the IL-33 high expression group was significantly increased(χ^(2)= 12. 554,P = 0. 000). Lymph node metastasis,TNM staging and IL-33 were independent risk factors for the recurrence of CRC patients. Conclusion The expression of IL-33 in the carcinoma tissues of CRC patients is significantly increased,and IL-33 is significantly associated with poor prognosis in patients with CRC.