超高效液相色谱法测定山楂原料、提取物和相关食品中金丝桃苷和异槲皮苷的含量

Determination of hyperoside and isoquercitrin in Crataegus pinnatifida Bge.raw materials,extracts and the related foods by ultra performance liquid chromatography

目的建立超高效液相色谱法测定山楂原料、提取物和相关食品中金丝桃苷和异槲皮苷的分析方法。方法采用ACQUITY UPLC BEH C_(18)柱(100 mm×2.1 mm,1.7μm),以甲酸乙腈-甲酸水为流动相进行梯度洗脱分离金丝桃苷和异槲皮苷,在紫外检测波长360 nm下进行检测,外标法定量。结果金丝桃苷和异槲皮苷的检出限(S/N=3)均为2 mg/kg,定量限(S/N=10)均为5 mg/kg;在0.5~10.0 mg/L的范围内线性关系良好,相关系数均为0.999;在原料、提取物和相关食品中,金丝桃苷和异槲皮苷的平均回收率分别为85.0%~108.0%和86.2%~105.0%,相对标准偏差(relative standard deviations,RSDs)分别为1.6%~5.7%和1.1%~3.9%。结论本方法操作简便、快速、分离度和准确度高,可用于山楂原料、提取物和相关食品的质量控制。

Objective To establish a method for the determination of hyperoside and isoquercitrin in Crataegus pinnatifida Bge.raw materials,extracts and the related foods by ultra performance liquid chromatography.Methods Hyperoside and isoquercitrin were separated on an ACQUITY UPLC BEH C_(18)column(100 mm×2.1 mm,1.7μm),eluted by formic acid-acetonitrile solution and formic acid-water solution,identified by ultraviolet detector at 360 nm and quantified by external standard method.Results As for hyperoside and isoquercitrin,the limits of detection(S/N=3)and the limits of quantification(S/N=10)were both 2 and 5 mg/kg respectively;the linear relationship was good in the ranges of 0.5-10.0 mg/L,and the correlation coefficients were 0.999,the average recoveries of hyperoside and isoquercitrin in Crataegus pinnatifida Bge.raw materials,extracts and the related foods were 85.0%-108.0%and 86.2%-105.0%,and the relative standard deviations(RSDs)were 1.6%-5.7%and 1.1%-3.9%,respectively.Conclusion This method is simple,rapid,high separation and accuracy,and can be used for the quality control of Crataegus pinnatifida Bge.raw materials,extracts and the related foods.