摘要
本文分别采用盐酸沉淀前处理方法和亚铁氰化钾沉淀前处理方法提取乳粉中的牛磺酸,并采用丹磺酰氯柱前衍生高效液相色谱法对乳粉中的牛磺酸含量进行测定。结果表明采用亚铁氰化钾沉淀法所得的谱图存在的杂质峰对牛磺酸谱峰产生干扰,造成牛磺酸谱峰出现肩峰影响测试结果。而采用盐酸沉淀法所得液相色谱谱图出现的杂质峰对牛磺酸谱峰不造成干扰。
Taurine in milk powder was extracted with hydrochloric acid or potassium ferrocyanide.The content of taurine was determined by high performance liquid chromatography(HPLC)with dansulfonyl chloride as precolumn derivatization.The shoulder peak may affect the test results when using potassium ferrocyanide as the precipitation.While the impurity peaks make no differences in the spectrogram when using hydrochloric acid as the precipitation.
作者
郁蕉竹
林洋
张宏宏
王韵
刘鹏
YU Jiao-zhu;LIN Yang;ZHANG Hong-hong;WANG Yun;LIU Pen(Shenyang Food and Drug Inspection Institute,Shenyang 110036,China;Shenyang Jianzhu University,Shenyang 110036,China)
基金
辽宁科技厅自然基金项目(2020-BS-158)。
