基于同位素相对标记与绝对定量技术结合质谱技术的幼年特发性关节炎血清蛋白质组学研究

Serum proteomics of juvenile idiopathic arthritis based on iTRAQ combined with mass spectrometry

目的应用同位素相对标记与绝对定量技术(iTRAQ)分析幼年特发性关节炎(JIA)患者与健康人群的血清差异蛋白。方法收集JIA患者血清18例、健康体检者20例、类风湿关节炎(RA)患者44例,采用iTRAQ标记联合纳升液相色谱-串联质谱筛选JIA患者的差异蛋白,并进行生物信息学分析。结果相对于健康体检组,JIA组共鉴定差异蛋白106个,其中49个表达上调,57个表达下调;JIA组和RA组中有26个差异蛋白均表达上调,37个表达下调。通过GO富集分析和KEGG通路分析,结果表明血液凝溶系统、免疫系统、脂质代谢与JIA的发病机制密切相关。通过STRING分析得到研究JIA机制的PPI网络的关键节点AHSG、APOH、脂联素等多个候选蛋白。结论本研究分析了JIA患者的血清蛋白质组,为JIA的诊断、JIA发病机制的探讨提供了实验依据。

Objective To a analyze serum differential proteins in patients with juvenile idiopathic arthritis(JIA).Methods Serum differential proteins were analyzed in 18 JIA patients(n=18),and 20 healthy peoplepopulation(n=20)and rheumatoid arthritis patients(n=44,RA)by iTRAQ combined with nano LC-MS/MS.Results Compared with the healthy group,the JIA group identified a total of 106 differential proteins were identified in the JIA group,with 49 up-regulated and 57 downregulated.A total of 63 proteins differential expressed in both JIA and RA group,with 26 up-regulated and 37 down-regulated.The blood coagulation and fibrinolysis system,immune system and lipid metabolism were closely related to the pathogenesis of JIA.AHSG and other proteins are candidate proteins for studying JIA mechanism as key nodes of protein-protein interaction network.Conclusions The serum differential proteins of JIA have been obtained successfully by proteomics technology,which provides a theoretical basis for exploring the diagnosis and pathogenesis of JIA.