郏县红牛FGF13基因拷贝数变异及其与生长性状关联性分析

Copy Number Variation of FGF13 Gene and Its Association with Growth Traits in Jiaxian Red Cattle

本研究旨在检测郏县红牛成纤维生长因子13(fibroblast growth factor 13,FGF13)基因的拷贝数变异(copy number variation,CNV)及其与生长性状之间的相关性分析。本研究利用实时定量PCR(real-time quantitative PCR,qPCR)技术,以57头郏县红牛为研究对象,检测FGF13基因CNV,并利用皮尔逊相关分析进行FGF13基因拷贝数与郏县红牛生长性状的关联分析。结果表明,郏县红牛FGF13基因的相对拷贝数高于对照组秦川牛(P<0.05),在郏县红牛群体中,FGF13基因CNV位点存在拷贝数增加Gain,拷贝数正常Normal和拷贝数减少Loss三种类型;关联分析结果显示,FGF13基因的相对拷贝数与郏县红牛的体斜长呈显著的正相关(P<0.05)。该研究结果表明,FGF13基因的CNV位点与郏县红牛的生长性状显著相关,可作为分子标记应用于郏县红牛的分子标记辅助选择育种工作中。

This study was to detect the copy number variation(CNV)of fibroblast growth factor 13(FGF13)gene in Jiaxian Red cattle and to analyze its association with growth traits.57 Jiaxian Red cattle were selected as objects and qPCR was used to detect CNV of FGF13 gene among them.Additionally,Pearson correlation analysis was applied to analyze association between CNV locus and growth traits.As a result,the relative copy number of FGF13 gene in Jiaxian Red cattle was significantly more than that in control group,Qinchuan cattle(P<0.05).There were three types of relative copy number of FGF13 gene in Jiaxian Red cattle,including Gain,Median,and Loss.Association analysis indicated that CNV of FGF13 gene was significantly positive correlated with body length(P<0.05).This study showed that CNV within bovine FGF13 gene was significantly related to growth traits in Jiaxian Red cattle.So it could be used as a candidate DNA marker in the marker-assisted selection of Jiaxian Red cattle.