脑梗死方急性毒性实验研究

An experimental study of the acute toxicity of cerebral infarction prescription

目的:研究脑梗死方给药后小鼠急性毒性反应情况,为临床安全用药提供参考。方法:将昆明(KM)小鼠40只随机分为空白组与给药组,每组各20只。给药组以药物最大浓度(11.68 g/ml)、最大体积(40 mg/kg)灌胃脑梗死方浓缩液,给药1次,对照组灌胃等量0.9%氯化钠注射液。连续16 d观察小鼠一般状态、饮食量、饮水量、体质量和死亡等情况,16 d后处死小鼠,测定血清尿素氮(BUN)、总胆固醇(CHO)、白蛋白(ALB)、总蛋白(TP)、葡萄糖(GLC)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、血小板总数(PLT)、红细胞计数(RBC)、白细胞计数(WBC)水平,并计算脏器系数。结果:给药组小鼠无中毒死亡情况,一般状态良好。2组饮食量、饮水量、体质量、BUN、CHO、ALB、TP、GLC、ALT、AST、ALP、PLT、RBC、WBC含量比较,差异均无统计学意义(P>0.05)。2组肝脏系数比较,差异有统计学意义(P<0.01)。结论:脑梗死方无明显的毒性作用,可为临床安全用药提供参考依据。

Objective: To investigate acute toxicity in mice after the administration of cerebral infarction prescription, and to provide a reference for safe medication in clinical practice.Methods: A total of 40 Kunming mice were randomly divided into blank group and administration group, with 20 mice in each group.The mice in the administration group were given the concentrated solution of cerebral infarction prescription once at the maximum concentration of 11.68 g/ml and the maximum volume of 40 mg/kg by gavage, and those in the control group were given an equal volume of 0.9% sodium chloride injection by gavage.The mice were observed in terms of general status, food intake, water consumption, body weight, and death for 16 consecutive days, and the levels of related serum markers were measured, including blood urea nitrogen(BUN),total cholesterol(CHO),albumin(Alb),total protein(TP),glucose(GLC),alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),platelet count(PLT),red blood cell count(RBC),and white blood cell count(WBC).Organ coefficient was also calculated.Results: No mice in the administration group died due to toxicity, and all mice had a good general status.There were no significant differences between the two groups in food intake, water consumption, body weight, BUN,CHO,ALB,TP,GLC,ALT,AST,ALP,PLT,RBC,and WBC(P>0.05),and there was a significant difference in the organ coefficient of the liver between the two groups(P<0.01).Conclusion: Cerebral infarction prescription has no obvious toxicity, which provides a reference for safe medication in clinical practice.