重水对萎缩芽孢杆菌芽孢萌发的影响

Effect of Deuterium Oxide on Bacillus atrophaeus Spore Germination

本研究快报报道了重水对细菌芽孢的萌发及其可培养性的抑制作用.在常温条件下,用L-丙氨酸触发细菌芽孢的萌发,并用Tb-DPA荧光法、相差显微镜观测法和光密度测定法监测萌发过程,用最终萌发水平、萌发半期、萌发速度3个参数来表征萌发过程.除此之外,我们还用菌落形成单位的个数来评估萌发后芽孢的可培养性.结果表明,重水对整个萌发过程有抑制作用,同时降低了萌发后芽孢的可培养性,但对最终萌发水平无影响.我们推测这是因为重水增强了一些芽孢特异性信号蛋白的稳定性.

This short communication is to report the inhibitory effect of deuterium oxide(D;O)on germination and culturability of bacterial spore.Germination was triggered by L-alanine under ambient condition and in situ monitored by Tb;-dipicolinic acid assay,phase contrast microscopy and absorbance measurement.Germination kinetics were characterized by final germination level,germination half time,and germination speed.The culturability of germinated spores was evaluated by enumerating colony-forming units.Results revealed that D;O retarded the whole germination process and reduced the culturability but didn’t affect the final germination level.We postulate that D;O enhanced the stability of some spore-specific signaling proteins.