瓜蒌子治疗Ⅱ型糖尿病的分子作用机制的网络药理学

Mechanism of Trichosanthis Semen Treating Type 2 Diabetes Based on Network Pharmacology

目的:运用网络药理学研究方法,探讨瓜蒌子治疗Ⅱ型糖尿病的可能分子作用机制。方法:通过BATMAN-TCM、Gene Cards、Uniprot、Swiss Target Prediction、Gene Cards等数据库检索Ⅱ型糖尿病相关靶点;建立瓜蒌子-活性成分-靶点-Ⅱ型糖尿病作用网络,并使用R语言数据库进行基因本体(GO)分析和京都基因与基因组百科全书(KEGG)通路富集分析。结果:筛选出瓜蒌子8个成分和330个预测靶点,Ⅱ型糖尿病11531个疾病靶点;通过韦恩图得到瓜蒌子和Ⅱ型糖尿病的共同靶点为252个,这些共同靶点涉及调节神经递质水平、调节胰岛素分泌、线粒体、离子通道活性、神经递质受体活性等生物学过程;生物学通路包括支链氨基酸代谢、视黄醇代谢、cAMP信号通路、cGMP-PKG信号通路等38条信号通路。结论:瓜蒌子可能是通过调节神经递质水平,以及改善神经递质受体活性,从而促进胰岛素分泌等机制治疗Ⅱ型糖尿病。

Objective:To explore the molecular mechanism of Trichosanthis Semen in the treatment of type 2 diabetes(T2D)based on network pharmacology.Methods:The targets and potential active ingredients of Trichosanthis semen were screened by BATMAN-TCM database,and the targets of type 2 diabetes(T2D)were predicted through Gene Cards database.And the targets Trichosanthis semen in the treatment of T2D were predicted by Venny2.1,Cytoscape software was used to construct the network of Trichosanthis semen-active ingredient-potential target-T2D and the protein interaction network,and the action targets of Trichosanthis semen were analyzed through using R database including the GO biological function analysis and KEGG pathway enrichment analysis.Result:A total of 8 active ingredients and 330 potential targets of Trichosanthis semen combined with T2D were obtained from BATMAN-TCM,11531 type 2 diabetes targets were retrieved from disease database.Then 252 common targets were obtained.Biological processes were related to neurotransmitter levels,regulation of insulin secretion,mitochondria,ion channel activity,activity of neurotransmitter receptors,etc.The 38 biological pathways mainly included branched chain amino acid metabolism,retinol metabolism,cAMP signaling pathway,cGMP-PKG signaling pathway and so on.Conclusion:Trichosanthis semen may play the anti-type 2 diabetes role by regulating neurotransmitter levels,and improve the activity of neurotransmitter receptors,thereby promoting insulin secretion for type 2 diabetes treatment,which may provide guidance for further experimental research.