地榆中酚酸类化合物对小鼠烫伤创面的影响

Effects of Phenolic Acids in Sanguisorba Officinalis on Scalded Wounds in Mice

目的:探究地榆醇提取物对小鼠深Ⅱ度烫伤创面的治疗作用及对转化生长因子-β1(TGF-β1)、血管内皮生长因子A(VEGF-A)和白细胞介素-6(IL-6)水平的影响。方法:小鼠随机分成为阴性对照组、地榆组和阳性对照组,烫伤后给药,1次/d,连续21 d。在烫伤后3、7、14、21 d取材,测定各组小鼠烫伤创面愈合率;在烫伤后第5、10、15 d时取样测定各组小鼠血清中TGF-β1、VEGF-A和IL-6的含量。结果:在创面愈合方面,地榆醇提取物组和阳性对照组均优于阴性对照组(p<0.01)。地榆醇提取物组和阳性对照组在第5 d时显著性升高TGF-β1和VEGF-A的含量(p<0.01),第10 d时可显著降低IL-6的水平(p<0.01)。结论:地榆醇提取物对小鼠深Ⅱ度烫伤具有促进创面愈合作用,其机制可能与早期升高TGF-β1和VEGF-A,降低血清中IL-6的含量有关。

Objective:To observe the wound healing effect of Sanguisorba officinalis L.extract on deepⅡdegree burn and the effect of transforming growth factor-β1(TGF-β1),vascular endothelial growth factor A(VEGF-A)and interleukin 6(IL-6)levels in serum.Methods Rats were randomly divided into negative control group,Sanguisorba officinalis L.extract group and positive control group.The drug was adopted for wounds once everyday for 21 days.Samples were collected at 3^(rd),7th,14^(th),21^(st) day after burn to determine the wound healing rate.Meanwhile,histopathological analysis was performed using tissue HE slices at 5^(th),10^(th),15^(th),20^(th) day after burn.The content of serum TGF-β1,VEGF-A and IL-6 in rats were detected by ELISA at 5^(th),10^(th),15^(th).Results Both Sanguisorba officinalis L.extract and positive control groups were superior to the negative control group in the healing rate.The content of TGF-β1 and VEGF-A was significantly higher than those in negative control group(p<0.01)at 5th.However,the level of IL-6 decreased 10 d after treatment with negative control group(p<0.01).Conclusion Sanguisorba officinalis L.extract improved the wound healing of scalds.It is speculated that this effect is achieved by up-regulating the level of TGF-β1 and VEGF-A at the early stage and inhibiting the release of inflammatory cytokines IL-6.