HPLC同时测定地榆中没食子酸和金丝桃苷的含量

Simultaneous Determination of the Contents of Gallic Acid and Hyperoside in Sanguisorba Officinalis L.by HPLC

目的:建立HPLC法同时测定地榆药材中没食子酸和金丝桃苷的含量方法。方法:采用Eclipse XDB-C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇-0.2%磷酸水为流动相,梯度洗脱,采用280、370 nm双波长,流速为1 mL·min^(-1);柱温为常温。结果:没食子酸和金丝桃苷的线性范围分别为23.00~115.00μg·mL^(-1)(r=0.9998),33.00~165.00μg·mL^(-1)(r=0.9991),平均加样回收率分别为98.88%(RSD=1.98%)和102.02%(RSD=0.48%)。结论:该方法操作简便,可作为地榆药材中没食子酸和金丝桃苷的含量测定方法。

Objective:To establish a simultaneous determination of the contents of gallic acid and hyperoside in Sanguisorba officinalis L.by HPLC.Methods:Eclipse XDB-C_(18) column was used to separate gallic acid and hyperoside in Sanguisorba officinalis L.Mobile phase A was methanol and mobile phase B was 0.2%H_(3)PO_(4) solution.It was eluted according to gradient concentration.The detection wavelength was 280 nm and 370 nm.The flow rate was at 1.0 mL·min^(-1) and the column temperature were set normal atmospheric temperature.Results:The linear ranges of gallic acid and hypericin were 23.00~115.00μg.ml^(-1) and 33.00~165.00μg·mL^(-1),respectively.The average recoveries were 98.88%(RSD=1.98%)and 102.02%(RSD=0.48%).Conclusion:The method is simple and can be used for the determination of gallic acid and hypericin in Sanguisorba officinalis L.