酸性乙醇提取黄柏中小檗碱的工艺优化

Optimization of Extraction Process of Berberine from Phellodendron Amurense by Acid Ethanol

目的:优化酸性乙醇提取黄柏中小檗碱的工艺。方法:采用酸性乙醇对黄柏中的小檗碱进行提取。通过正交试验比较酸性乙醇比例、料液比、提取温度和提取时间对小檗碱提取量的影响,筛选出酸性乙醇提取小檗碱的最佳提取工艺参数。结果:超声辅助提取黄柏中小檗碱最佳提取工艺参数为:硫酸与乙醇比例1︰1.5,料液比1︰12,提取温度60℃,提取时间30 min。结论:该提取工艺操作简单可行,可用于黄柏中小檗碱的提取。

Objective:To optimize the extraction process of berberine from Phellodendron amurense by acid ethanol.Methods:The extraction of berberine from Phellodendron amurense with acid alcohol was studied.The rational extraction process of berberine from Phellodendron amurense by acid-ethanol ratio,material-liquid ratio,extraction temperature and extraction time were selected by orthogonal extraction.Result:The best extracting condition of berberine from Phellodendron amurense was as follows:ratio of HSO_(4) to ethanol was 1∶1.5,ratio of lipuid to solid was 12∶1,extraction temperature was 60℃ and extraction time was 30 min.Conclusion:The extraction process is simple.It can be used as the extraction process of berberine from Phellodendron amurense.