鄂西北秃杉侧枝扦插试验

Lateral Branch Cutting of Taiwania flousiana in Northwest Hubei Province

为了筛选适合秃杉扦插繁殖的方法,以6~7年生长健壮秃杉的1~2年生侧枝为插穗材料,将5种不同质量浓度的ABT1号生根粉(1000、500、250、100、50 mg·L^(-1))分别以5个不同处理时间(5、15、30、60、360 min)进行浸泡处理,随后于圃地进行扦插试验,12个月后调查扦插育苗效果。结果表明:不同质量浓度、不同处理时间的扦插效果有显著差异。所有处理中,愈伤组织诱导率最高的处理为使用质量浓度为250 mg·L^(-1)的ABT1号生根粉处理15 min,其愈伤组织诱导率达83.33%;愈伤组织生长最佳的处理为使用质量浓度为500 mg·L^(-1)的ABT1号生根粉处理30 min、质量浓度为250 mg·L^(-1)的ABT1号生根粉处理5 min,其愈伤组织的直径均达到0.61 cm;生根率最高的处理为使用质量浓度为250 mg·L^(-1)的ABT1号生根粉处理360 min及使用质量浓度为1000 mg·L^(-1)的ABT1号生根粉处理15 min,其生根率均为46.67%;生根数量最多的处理为使用质量浓度为250 mg·L^(-1)的ABT1号生根粉处理360 min,平均生根数量为2.43条;根系长度生长最佳的处理为使用质量浓度为500 mg·L^(-1)的ABT1号生根粉处理360 min,其平均根长为10.04 cm;苗高生长最佳的处理为使用质量浓度为500 mg·L^(-1)的ABT1号生根粉处理5 min,其苗高为7.88 cm;地径生长最佳的处理为使用质量浓度为1000 mg·L^(-1)的ABT1号生根粉处理5 min,其地径生长量为0.58 cm;新梢生长最佳的处理为使用质量浓度为500 mg·L^(-1)的ABT1号生根粉处理30 min,其长度达到3.93 cm。最适宜秃杉扦插繁殖的处理为使用质量浓度为250 mg·L^(-1)的ABT1号生根粉处理360 min,此时扦插成活率最高,生根数量最多,苗木生长较好。

In order to select suitable cutting propagation methods of Taiwania flousiana,1-2-a lateral branches of 6-7-a robust T.flousiana were used as cutting materials.ABT1 rooting powder was set at five different concentrations(1000,500,250,100 and 50 mg/L),and soaked for five different times(5,15,30,60 and 360 min),and then the cutting experiment was carried out in the nursery for 1 a,and 12 months later,the effect of cuttage seedling was investigated.There were significant differences in cutting effect between different concentrations and different time treatments.Among all the treatments,the highest callus induction rate was 250 mg/L ABT1 for 15 min,and the callus induction rate was 83.33%;the best treatment for callus growth was 500 mg/L ABT1 for 30 min or 250 mg/L ABT1 for 5 min,with the diameter of 0.61 cm;the highest rooting rate was 250 mg/L ABT1 for 360 min and 1000 mg/L for 15 min,the rooting rate was 46.67%;the treatment with the largest number of rooting was 250 mg/L ABT1 for 360 min,the average rooting number was 2.43;the best treatment for root length was 500 mg/L ABT1 for 360 min,the average root length was 10.04 cm;the best treatment for seedling height growth was 500 mg/L ABT1 for 5 min,the height was 7.88 cm,and the best treatment for ground diameter growth was 1000 mg/L ABT1 for 5 min,with the diameter of 0.58 cm;the best treatment for new shoots growth was 500 mg/L ABT1 for 30 min,reaching 3.93 cm.Therefore,the most suitable cutting propagation treatment was 250 mg/L ABT1 for 360 min.By the treatment,cutting survival rate and rooting quantity were the highest,coupled with superior seedling growth.