摘要
目的:优选三元两相溶剂提取中药虎杖中白藜芦醇的工艺条件。方法:采用正交试验和单因素试验,通过薄层扫描外标法进行含量测定,以白藜芦醇提取率和纯度为考察指标,优选提取工艺条件。结果:最佳提取工艺为:丙酮︰水(7︰3)溶液按照1︰10料液比超声提取两次,每次15 min,调粗提液pH至8.5,加入3/5体积二氯甲烷,充分振摇,35℃下静置分层40 min。工艺验证结果表明经一步萃取后白藜芦醇纯度可达46%,提取率为4.1‰,RSD分别为0.97%和2.19%。结论:优选工艺简单高效,稳定可行。
Objective:To optimizing the technology condition of ternary two-phase liquid–liquid extraction for resveratrol from Polygonum Cuspidatum.Method:The content of resveratrol was determined by TLC,and the extraction yield and purity of resveratrol were regarded as the index.Orthogonal test and a single factor test were used to optimize the extraction process of resveratrol in Polygonum cuspidatum.Results:The optimum extraction condition is that the ratio of material to liquid(acetone/water=7/3)is 1︰10,the extraction is twice and 15 minutes per time by ultrasonic,the pH of the solution is adjusted to 8.5 and the volume of dichloromethane is 3/5 of acetone/water,the phase separation temperature is at 35℃for 40 min.Results shows that only for one extraction step the purity of resveratrol is 46%,and the extraction yield is 4.1‰,the RSD were 0.97%and 2.19%respectively.Conclusion:The optimum technology condition is uncomplicated,efficient,stable and feasible.
作者
向玲丽
高俊飞
Xiang Lingli;Gao Junfei(School of Health,Guangzhou Huaxia Vocational College,Guangzhou 510935;Research and Development Centre,Guangdong Guobiao Pharma-Tech Co.,Ltd.,Guangzhou 510935,China)
出处
《广东化工》
CAS
2021年第20期24-26,共3页
Guangdong Chemical Industry
基金
广州华夏职业学院自然科学基金项目“中药虎杖中白藜芦醇美白保湿面膜的制备与性能评价”(ZK20200105)。
关键词
虎杖
白藜芦醇
正交设计
三元两相溶剂
提取工艺
polygonum cuspidatum
resveratrol
orthogonal design
ternary two-phase solvent
extraction technology