摘要
成簇间隔短回文重复序列(CRISPR-Cas9)技术是研究细菌和古生菌重复序列得到的可抵抗病毒或质粒入侵获得性免疫防御技术,是一种由sgRNA指导Cas蛋白对目的基因定性定向编辑的技术。文章介绍CRISPR-Cas9技术原理和功能,整理CRISPR-Cas9技术对猪生产性能的影响,综述在猪抗病育种中的应用以及器官移植相关的最新研究。
Clustered interval short palindromic repeats(CRISPR-Cas9)technology is an acquired immune defense technology that can resist virus or plasmid invasion by studying the repeated sequences of bacteria and archaeo.It is a kind of sgRNA to guide Cas protein to qualitative target genes.Directional editing technology.The article introduces the principle and function of CRISPR-Cas9 technology,sorts out the impact of CRISPR-Cas9 technology on pig production performance,and reviews its application in pig breeding for disease resistance and the latest research related to organ transplantation.
作者
房元杰
张晓爱
魏文康
刘春朋
Fang Yuanjie;Zhang Xiaoai;Wei Wenkang;Liu Chunpeng(College of Animal Science and Technology,Zhongkai University of Agriculture and Engineering,Guangdong Guangzhou 510225;Agro-biological Gene Reasearch Center,Gunagdong Academy of Agricultural Sciences,Guangdong Guangzhou 510640)
出处
《现代畜牧兽医》
2021年第11期92-96,共5页
Modern Journal of Animal Husbandry and Veterinary Medicine
基金
广东省自然科学基金(2018A030310195)
广州市科技计划项目一般项目(201804010338)。
