浙江省主栽水稻品种抗稻瘟病基因的分子检测

Molecular Detection of Blast Resistance Genes in Main Rice Varieties of Zhejiang Province

稻瘟病是水稻的三大重要病害之一。利用稻瘟病抗病基因进行新品种培育是控制稻瘟病发生最经济有效的措施,对水稻安全生产具有重要意义。本研究在鉴定63个浙江省主栽水稻品种的叶瘟和穗颈瘟抗性水平的基础上,利用Pi1、Pi5、Pi2、Pi9、Pia、Pizt、Pigm、Pib、Pik和Pikh等10个已克隆抗稻瘟病基因的特异分子标记,对这63个品种的抗稻瘟病基因组成进行了鉴定,并进一步分析了抗病基因与稻瘟病抗性之间相关性。抗性鉴定结果显示,63个浙江省主栽水稻品种中,抗叶瘟品种占66.7%、抗穗瘟品种占50.8%。抗稻瘟病基因分析显示,有60个主栽品种聚合了多个抗病基因,含有Pizt和Pib基因的品种45个、含有Pi2和Pi5基因的品种43个,此外,抗病基因Pia、Pik、Pi1、Pi2、Pizt、Pikh、Pi5及Pib在浙江省主栽品种中分布频率整体较高,而Pigm和Pi9分布频率很低,仅3.2%。本研究揭示了浙江省主栽水稻品种抗稻瘟病基因的组成及抗稻瘟病基因对稻瘟病抗性提高的贡献,为浙江省抗病新品种的培育提供参考。

Rice blast is one of the three most important rice diseases.Using rice blast resistance genes to breed new varieties is the most economical and effective measure to control the occurrence of rice blast,and is of great significance to the safety of rice produc-tion.Based on the identification of the leaf blast and panicle blast resistance levels of 63 main rice varieties in Zhejiang Province,the specific molecular markers of 10 cloned rice blast resistance genes Pi1,Pi5,Pi2,Pi9,Pia,Pizt,Pigm,Pib,Pik and Pikh were used to identify the gene composition of these 63 varieties for resistance to rice blast,and further analyzed the correlation between resistance genes and resistance to rice blast.The resistance identification results showed that among 63 varieties,the leaf blast resistant varieties accounted for 66.7%and the ear blast resistant varieties accounted for 50.8%.Rice blast resistance genes identified showed that 60 of63 the main cultivated varieties had more than one resistance genes,45 of which contained Pizt and Pib genes,and 43 varieties contained Pi2 and Pi5 genes.In addition,the distribution frequency of disease-resistant genes Pia,Pik,Pi1,Pi2,Pizt,Pikh,Pi5 and Pib among the main rice varieties in Zhejiang Province is relatively high,while the distribution frequency of Pigm and Pi9 is very low,only 3.2%.This study revealed the composition of the rice blast resistance genes of the main rice varieties in Zhejiang Province and the contribution of the rice blast resistance genes to the improvement of resistance to rice blast,provided an important reference for the breeding of new disease-resistant varieties in Zhejiang Province.