京尼平改性牛心包眼巩膜生物补片的结构表征及性能评价

Structure characterization and performance evaluation of modified bovine pericardial scleral biological patch with genipin

背景:目前生物补片在眼后巩膜加固术中的应用受到广泛关注。眼巩膜生物补片的结构和性能直接关系到其植入机体后的成功与否,因而对其进行结构表征和性能评价至关重要。目的:探讨京尼平改性牛心包眼巩膜生物补片的结构及性能。方法:采用光学显微镜、扫描电镜、红外光谱仪和X射线衍射仪表征新鲜组、脱细胞组和京尼平改性组牛心包的形貌结构,通过测定力学性能、含水率和热稳定性等表征其理化指标,通过遗传毒性实验检测京尼平改性组牛心包的生物相容性。结果与结论:(1)组织学与扫描电镜观察结果显示,脱细胞处理有效去除了材料中的细胞成分;脱细胞和京尼平改性处理后的胶原纤维结构保存完好有序,没有破坏胶原纤维的稳定结构;(2)红外光谱仪和X射线衍射仪表征结果显示,京尼平改性组牛心包胶原纤维结构中出现了更多的酰胺基团;新鲜组和脱细胞组牛心包的晶相不完整、结晶较差,京尼平改性组的结晶度有所提高;(3)京尼平改性组牛心包的拉伸强度显著大于脱细胞组(P<0.05),断裂伸长率显著小于脱细胞组(P<0.05);(4)京尼平改性组牛心包的含水率与脱细胞组比较差异无显著性意义(P>0.05);(5)京尼平改性组牛心包的热变性温度大于脱细胞组;(6)京尼平组的体外哺乳动物染色体畸变实验结果为阴性;(7)结果表明,京尼平改性牛心包眼巩膜生物补片具有良好的理化性能和生物相容性。

BACKGROUND:At present,the application of biological patch in posterior scleral reinforcement has received extensive attention.The structure and performance of the biological scleral mesh are directly related to the success rate of the implant,so it is very important to characterize the structure and evaluate the performance of the biological mesh.OBJECTIVE:To investigate the structure and properties of the modified bovine pericardial scleral biological patch with genipin.METHODS:The structures of fresh group,acellular group and genipin group were characterized by optical microscope,scanning electron microscopy,infrared spectrometer,and X-ray diffraction.Their physical index was evaluated through the determination of mechanical properties,water content rate and thermal stability.In addition,the biocompatibility of bovine pericardium patch in genipin group was evaluated by genetic toxicity test.RESULTS AND CONCLUSION:(1)The results of histology and scanning electron microscopy showed that the cellular components in the material were effectively removed by acellular treatment.After acellular treatment and genipin modification,the collagen fiber structure was well preserved and orderly without destroying the stable structure of the collagen fiber.(2)Infrared spectrometer and X-ray diffraction demonstrated that there were more amide groups in the collagen fiber of bovine pericardium in genipin group.In addition,the crystal phase of bovine pericardium in fresh and acellular groups was incomplete and the crystallization was poor,while its crystallinity of genipin group was improved.(3)Tensile strength of bovine pericardium in genipin group was significantly higher than that of acellular group(P<0.05);and elongation at break was significantly lower than that in acellular group(P<0.05).(4)There was no significant difference in the water content rate between genipin group and acellular group(P>0.05).(5)The temperature of thermal denaturation in genipin group was higher than that in acellular group.(6)The in vitro mammalian chromosome aberration test in genipin group showed negative result.(7)The results suggested that modified bovine pericardium patch for posterior scleral reinforcement with genipin had good physical and chemical properties and biocompatibility.