副干酪乳杆菌HCS17-040的筛选鉴定及其益生特性

Screening of Lactobacillus Paracasei HCS17-040 with triglyceride tunction and study on its probiotic characteristics

目的:获得具有降甘油三酯功能的乳酸菌菌株。方法:从母乳及顺产的健康婴儿粪便中筛选分离乳酸菌菌株,采用单试剂法乳酸菌(GPO-PAP法)测定其甘油三酯去除能力。从中选择甘油三酯去除能力强的菌株进行形态学鉴定、生理生化及16S rDNA鉴定,并测定其耐受酸、胆盐和胃肠液的能力。结果:筛选分离出一株副干酪乳杆菌(Lactobacillus paracasei),命名为HCS17-040(CGMCC No 19747),其甘油三酯下降率达(42.80±1.79)%;该株菌在pH 3.0和pH 2.0的环境中培养17 h后存活率分别为89.39%和87.18%;在3 g/L和5 g/L的胆盐浓度环境中培养17 h后存活率分别为94.75%,92.97%;在人工胃液中作用3 h,存活率为93.93%;在人工肠液中作用4 h,存活率高达99.06%。结论:副干酪乳杆菌HCS17-040对甘油三酯具有一定的去除能力,且具有良好的耐酸、胆盐及人工胃肠液的能力。

Objective:This study focuses on obtaining a Lactobacillus strain for degrading triglyceride.Methods:Lactocillus was isolated from human breast milk and the faeces of healthy infants in this study,and their triglyceride degradability was determined by single reagent(GPO-PAP)method.The strains with strong degradation ability of triglyceride were selected for morphological,physiological,biochemical and 16S rDNA identification,and their tolerance to acid,bile salt and gastrointestinal juice was determined.Results:A strain of Lactobacillus Paracasei was isolated and then named as HCS17-040(CGMCC No 19747),and its degradation rate of triglyceride was(42.80±1.79)%;the survival rates of the strain were 89.35%and 87.18%after 17 h culture in pH 3.0 and pH 2.0,respectively,and then reached 94.75%and 92.97%after 17 h culture in 3 g/L and 5 g/L bile salts,respectively,the survival rate was 93.93%and 99.06%in artificial intestinal fluid for 4 hours.Conclusion:Lactobacillus Paracasei HCS17-040 has a certain ability to remove triglycerides,and has a good ability to resist acid,bile salt and artificial gastric and intestinal fluid.