HPLC法测定金酸萍颗粒中蒽醌类成分的含量

Determination of Anthraquinones in Jinsuanping Granules by HPLC

目的:建立中成药金酸萍颗粒中大黄素、大黄酚、大黄素甲醚3种蒽醌类成分的HPLC含量测定方法。方法:采用Thermo Hypersill GOLD C 18色谱柱(4.6 mm×250 mm,5μm);流动相为甲醇-0.1%磷酸水溶液液(85∶15);流速为1.0 mL/min;检测波长选择254 nm;柱温在25℃。结果:大黄素、大黄酚以及大黄素甲醚分别在相应的浓度范围内与峰面积的线性关系均良好(r≥0.9999);平均加样回收率分别为96.12%、104.22%和97.41%,RSD分别为2.47%、1.57%和1.96%。结论:本方法操作方便、准确、重现性好,可用于金酸萍颗粒的质量控制。

Objective An HPLC method was established for the simultaneous determination of three anthraquinones(emodin,chrysophanol and physcion)in Jinsuanping Granules(Siphonostegiae Herba,Rumicis acetosae Radix,Marsileae Herba).Method The Thermo Hypersill GOLD(4.6 mm×250 mm,5μm)was adopted and the mobile phase was methanol-0.1%phosphoric acid aqueous solution(85∶15,V/V)with a flow rate at 1.0 mL/min.The detection wavelength was set at 254 nm and the column temperature was kept at 25℃.Results Emodin,chrysophanol and physcion showed a good linear relationship with peak areas in the corresponding concentration range(r≥0.9999).Whose average recovery rates were 96.12%,104.22%and 97.41%,respectively,and the RSD was 2.47%,1.57%and 1.96%,respectively.Conclusion It’s a convenient,accurate and reproducible method,and can be used to control the quality of Jinsuanping Granules.