4种松花粉的成分鉴定和差异分析

Composition Identification and Difference Analysis of Four Pine Pollen

利用UPLC-MS/MS技术对马尾松Pinus massoniana、湿地松P.elliottii、火炬松P.taeda和湿加松P.elliottii×P.caribaea松花粉的代谢物成分进行了鉴定与分析。共鉴定到368种代谢物,主要为脂质类、有机酸类、氨基酸类、糖及醇类和黄酮类等化合物。差异统计分析结果表明,在4种松花粉组织中共鉴定到175种差异积累代谢物。分别有31、6、5和2种代谢物在马尾松、火炬松、湿加松和湿地松松花粉中显著高积累。其中原儿茶酸和神经酸在马尾松松花粉中特异性积累。8种必需氨基酸和绝大部分的不饱和脂肪酸和黄酮类化合物在4种花粉组织中的含量均无显著差异。综合表明4种松花粉均富含多种营养成分及高生物活性物质,可为相应的药物和保健产品提供原材料。

The metabolites compositions and content differences in the pollen of Pinus massoniana,P.elliottii,P.taeda and P.elliottii×P.caribaea were analyzed by UPLC-MS/MS.A total of 368 metabolites were identified,including the lipids,organic acids,amino acids,saccharides and alcohols,and flavonoids.The results of statistical analysis showed that total of 175 differential metabolites were identified in four pine pollen.Among them,31,6,5 and 2 metabolites were significantly high accumulation in the pollen of P.massoniana,P.taeda,P.elliottii×P.caribaea and P.elliottii,respectively.Especially,protocatechuic acid nervonic acid were specifically accumulated in the pollen of P.massoniana.However,there was no significant difference in the contents of most of the identified amino acids,unsaturated fatty acids and flavonods.The results showed that all of the four pine pollen were rich in various nutrients and bioactive substances,and could be used as raw materials for medicine and health care products.