野百合碱诱导大鼠肺动脉高压动物模型的制备

Preparation and determination of animal model of pulmonary hypertension induced by monocrotaline in rats

目的采用注射野百合碱(MCT)制备大鼠肺动脉高压模型,并对肺动脉压测定方法进行优化。方法通过ip MCT诱导建立大鼠肺动脉高压模型,通过测定大鼠肺血管阻力的方法来对大鼠右心室压进行测定,采用阳性药对照、血流动力学测定和组织学观察确定模型是否建立成功。结果 ip 50 mg/kg MCT 4周后大鼠右心室平均压、右心肥大指数对比对照组明显增高,观察病理组织切片,可见明显的内皮细胞损伤,分布不均,动脉管壁明显增厚,肺组织有大量的炎性细胞浸润,出现肺血管的重构,证实肺动脉高压动物模型造模成功。结论采用ip MCT可以建立稳定的大鼠肺动脉高压模型,且具有较高的存活率,同时建议采用改良的心导管测定方法测定大鼠右心室压力。

Objective To establish pulmonary hypertension model of rats by injection of monocrotaline(MCT), and to optimize measurement method of pulmonary arterial pressure. Methods Rat pulmonary hypertension model was established by ip MCT induction, and right ventricular pressure was measured by measuring pulmonary vascular resistance in rats. Positive drug control, hemodynamic measurement, and histological observation were used to determine whether the model was established successfully. Results Four weeks after ip MCT rat average pressure, right heart right ventricular hypertrophy index were increased compared to control group obviously, observed the pathological tissue slice, visible obvious endothelial cell damage, uneven distribution, the artery wall thickening obviously, there are a large number of inflammatory cells infiltration, lung tissue of pulmonary vascular reconstruction, confirmed that the pulmonary hypertension animal model building success. Conclusion Intraperitoneal injection of 50 mg/kg MCT can establish a stable rat model of pulmonary hypertension with a high survival rate, and it is recommended to use an improved cardiac catheterization method to measure right ventricular pressure in rats.