维生素E对妊娠中期高糖环境大鼠皮下脂肪组织中asprosin的表达影响

Effect of Vitamin E on Expression of Asprosin in Subcutaneous Adipose Tissue of Rats with High Glucose in Second Trimester of Pregnancy

目的:探讨维生素E对妊娠中期高糖环境大鼠皮下脂肪组织中asprosin的表达影响。方法:将妊娠中期高糖环境大鼠(n=21)随机平分为三组-模型组、吡格列酮与维生素E组。格列酮与维生素E组分别灌胃80 mg/kg的吡格列酮和5 mg/kg的维生素E,模型组灌胃等剂量的0.9%NaCl,1次/d,检测皮下脂肪组织中asprosin表达情况。结果:吡格列酮组与维生素E组给药第3 d、第7 d的血糖、体重低于模型组(P<0.05),维生素E组低于吡格列酮组(P<0.05)。吡格列酮组与维生素E组给药第7 d的皮肤组织超氧化物歧化酶(Superoxide dismutase,SOD)含量高于模型组(P<0.05),丙二醛(Malondialdehyde,MDA)含量低于模型组(P<0.05),吡格列酮组与维生素E组对比差异也都有统计学意义(P<0.05)。吡格列酮组与维生素E组给药第7 d的皮肤组织asprosin蛋白相对表达水平低于模型组(P<0.05),维生素E组低于吡格列酮组(P<0.05)。结论:维生素E在妊娠中期高糖环境大鼠的应用能抑制皮下脂肪组织中asprosin的表达,提高SOD活性,降低MDA的表达,从而降低大鼠的体重与血糖水平。

Objective:To investigate the effect of vitamin E on the expression of asprosin in the subcutaneous adipose tissue of rats in high glucose environment in the second trimester of pregnancy.Methods:A total of 21 rats in high glucose environment in the second trimester were randomly divided into three groups-model group,pioglitazone group and vitamin E group.Glitazone group and vitamin E group were intragastrically administered with 80 mg/kg pioglitazone and 5 mg/kg vitamin E,respectively;the model group was intragastrically administered with the same dose of 0.9%NaCl.All the rats were detected the expression of asprosin in the subcutaneous adipose tissue.Results:The blood glucose and body weight of the pioglitazone group and the vitamin E group were lower than those of the model group on the 3rd and 7th day of dosing(P<0.05),and the vitamin E group were lower than the pioglitazone group(P<0.05).The content of superoxide dismutase(SOD)in the skin tissues of the pioglitazone group and vitamin E group was higher than that in the model group(P<0.05),and the content of malondialdehyde(MDA)was lower than that in the model group(P<0.05),the difference between the pioglitazone group and the vitamin E group was also statistically significant on the 7th day of dosing(P<0.05).The relative expression levels of asprosin protein in the skin tissues of the pioglitazone group and the vitamin E group on the 7th day of dosing were lower than those in the model group(P<0.05),and the vitamin E group was lower than that in the pioglitazone group(P<0.05).Conclusion:The application of vitamin E in the rats with high glucose in the second trimester of pregnancy can inhibit the expression of asprosin in the subcutaneous adipose tissue,increase the activity of SOD,and reduce the expression of MDA,thus reducing the weight and blood glucose levels of rats.