医用五谷虫治疗糖尿病足的效果及其对Notch1 mRNA表达的影响

Therapeutic effect of medical maggot on diabetic foot ulcer and its influence on Notch1 mRNA expression

目的观察医用五谷虫对糖尿病足的疗效,并分析其可能的作用机制。方法选取2018年7月至2019年9月大连医科大学附属第二医院Wagner分级1~2级糖尿病足溃疡患者60例,按随机数字表法分为对照组(采用常规方法治疗)和试验组(采用常规方法联合医用五谷虫治疗),每组30例。试验组患者于治疗前和治疗第4天采用聚合酶链反应法检测创面Notch 1 mRNA表达量。比较两组治疗后21 d创面愈合率。结果试验组痊愈率明显高于对照组[100.00%(30/30)比76.67%(23/30)],差异有统计学意义(χ^(2)=7.925,P<0.01)。试验组治疗前和治疗第4天创面Notch 1 mRNA表达量比较差异无统计学意义(0.96±0.08比0.93±0.08,t=1.545,P>0.05)。结论医用五谷虫对于Wagner分级1~2级糖尿病足溃疡具有明显疗效,其作用机制可能与Notch 1信号通路无关。

Objective To observe the therapeutic effect of medical maggot on diabetic foot,and analyze its possible mechanism.Methods Sixty diabetic foot ulcer patients with Wagner grade 1 or 2 from July 2018 to September 2019 in Second Affiliated Hospital of Dalian Medical University were selected.The patients were divided into control group and experimental group by random digits table methods with 30 cases in each group.The control group was treated with traditional method,and the experimental group was treated with medical maggots.In experimental group,the wound surface Notch 1 mRNA expression before treatment and at the 4th day of treatment was detected by polymerase chain reaction method.The recovery rate 21 d after treatment was compared between 2 groups.Results The recovery rate in experimental group was significantly higher than that in control group:100.00%(30/30)vs.76.67%(23/30),and there was statistical difference(χ^(2)=7.925,P<0.01).In experimental group,there was no statistical difference in the wound surface Notch 1 mRNA expression between before treatment and the 4th day of treatment(0.96±0.08 vs.0.93±0.08;t=1.545,P>0.05).Conclusions Medical maggot has an obvious effect on Wagner grade 1 or 2 diabetic foot ulcers,but its mechanism maybe unrelated to Notch1 signaling pathway.