花粉块内花粉粒形态观察的扫描电镜样品制备方法

SEM Sample Preparation of Observation Pollen Grains Morphology in Pollinium

探讨在扫描电镜下观察花粉块内花粉粒的样品制备方法。以翅萼石斛(Dendrobium cariniferum Rchb.f.)的花粉块为材料,在常规扫描电镜样品制备的基础上,采用导电双面胶带拉断法,暴露出花粉块内部的花粉粒,使用扫描电镜进行观察。在扫描电镜下清晰观察到了花粉块内花粉粒的立体形态、大小和表面纹饰。此法简单易行,操作方便,进一步优化后可作为扫描电镜下观察花粉块内花粉粒形态的通用方法。

canning electron microscopy(SEM)had been widely used in the study of palynomorphology.However,it was difficult to directly observe the morphology of pollen grains in Pollinium.In this paper,westudied the sample preparation method for obvervation pollen grains in pollinium.The pollinium of Dendrobium cariniferum Rchb.f.was used as the material,based the conventional SEM sample preparation,the pollengrains inside the pollinium were exposed by the conductive double-sided tape breaking method and thenobserved by SEM.The stereoscopic shape,size and surface texture of pollen grains were observed clearly underthe SEM.This method was simple,easy to operate and can be used as a general method to observe themorphology of pollen grains in pollinia by SEM after further optimization.