棕榈酸对骨骼肌细胞脂质沉积的诱导作用

Effect of palmitic acid on induction of lipid deposition in skeletal muscle cells

目的:探讨棕榈酸(PA)对骨骼肌细胞脂质沉积的作用及其最佳作用浓度和时间。方法:在C2C12细胞生长至70%~80%时分为对照组和不同浓度(50、75、100和125μmol·L^(-1))PA组。不同浓度PA组细胞采用含不同浓度PA的生长培养基培养,对照组细胞不给予PA(0μmol·L^(-1)PA)。CCK-8法检测各组细胞增殖活性,定量检测细胞中甘油三酯(TG)水平,油红O染色观察细胞中脂滴形成情况,2%马血清诱导细胞分化并评价各组C2C12细胞分化状态。结果:与对照组比较,50、75和100μmol·L^(-1)PA组C2C12细胞增殖活性差异无统计学意义(P>0.05),125μmol·L^(-1)PA组C2C12细胞增殖活性明显降低(P<0.01),50、75和100μmol·L^(-1)PA组细胞中TG水平升高(P<0.01),100μmol·L^(-1)PA组C2C12细胞中TG水平升高最明显(P<0.01);与培养24 h比较,100μmol·L^(-1)PA培养48 h后,C2C12细胞中TG水平升高(P<0.01)。分化培养后,PA组和对照组C2C12细胞中均有较多肌管形成。结论:100μmol·L^(-1)PA作用48 h可有效促进C2C12细胞脂质沉积。

Objective:To explore the effect of palmitic acid(PA)on the lipid deposition in the skeletal muscle cells,and to discuss the optimum action concentration and time.Methods:The C2C12 cells were divided into control group and different concentrations(50,75,100,and 125μmol·L^(-1))of PA groups when the C2C12 cells grew to 70%-80%.The C2C12 cells in different concentrations of PA groups were given growth media containing different concentrations of PA,while the C2C12 cells in control group were not treated with PA(0μmol·L^(-1)PA).CCK-8 method was used to detect the cell proliferation activities of cells in various groups,the triglyceride(TG)levels in the C2C12 cells in various groups were detected,Oil Red O staining was used to observe the formation of lipid droplets in the C2C12 cells in various groups,and 2%horse serum was used to induce the cell differentiation and evaluate the differentiation of C2C12 cells in various groups.Results:Compared with control group,there were no significant differences in the proliferation activities of the C2C12 cells in 50,75,and 100μmol·L^(-1)PA groups(P>0.05),the proliferation activity of the C2C12 cells in 125μmol·L^(-1)PA group was decreased(P<0.01),the TG levels in the C2C12 cells in 50,75 and 100μmol·L^(-1)PA groups were increased(P<0.01),and the TG level in the C2C12 cells in 100μmol·L^(-1)PA group was increased significantly(P<0.01).Compared with cultured for 24 h,the TG level in the C2C12 cells after treated with 100μmol·L^(-1)PA for 48 h was increased(P<0.01).After differentiation and culture,more myotubes were formed in the C2C12 cells in PA groups and control group.Conclusion:The lipid deposition in the C2C12 cells could be effectively promoted after cultured with 100μmol·L^(-1)PA for 48 h.