熟鸡肉中沙门氏菌生长模型的建立及货架期预测

Growth modeling for Salmonella of cooked chicken and shelf life prediction

为了建立沙门氏菌的生长模型并预测货架期,以熟鸡胸肉为载体,接种沙门氏菌,在恒温条件下进行培养,采用Logistic模型、Gompertz模型及Ratkowsky二级模型进行拟合,并预测其货架期。结果表明,沙门氏菌在4℃条件下无明显生长,在8~35℃能够正常生长,且温度越高生长速率越快,迟滞期越短。两种一级模型拟合的决定系数R2均达到了0.99,Logistic模型拟合度更高。采用二级Ratkowsky模型拟合的决定系数R2为0.9641。预测了8、16、25、35℃等4个温度下的货架期,分别为10.794、9.154、4.099、1.724 h。货架期与最大生长速率的相关系数为-0.9926,与迟滞期的相关系数为0.9931。

In order to establish the growth model of Salmonella and predict the shelf life,cooked chicken breast meat was inoculated with Salmonella and cultured under constant temperature.Logistic model,Gompertz model and Ratkowsky secondary model were used to fit and predict the shelf life.The results showed that Salmonella did not grow significantly at 4℃,but could grow normally at 8~35℃,and the higher the temperature,the faster the growth rate and the shorter the hysteresis period.The determination coefficient R2 of the two first-order models reached 0.99,and the fitting degree of Logistic model was higher.The determination coefficient R2 fitted by two-stage ratkowsky model is 0.9641.The shelf life at 8,16,25 and 35℃were predicted to be 10.794,9.154,4.099 and 1.724 h respectively.The correlation coefficient between shelf life and maximum growth rate was-0.9926,and the correlation coefficient with hysteresis was 0.9931.