p16、Ki-67在不同宫颈病变组织中的表达及其水平检测在宫颈良恶性病变诊断中的效能

Expressions of p16 and Ki-67 in different cervical lesions and efficacies of their level detection in diagnosis of cervical benign and malignant lesions

目的:观察p16、Ki-67在不同宫颈病变组织中的表达及其水平检测在宫颈良恶性病变诊断中的效能。方法:回顾性分析2017年5月至2020年5月该院收治的90例宫颈病变患者的临床资料,术中留取病灶组织送检,经免疫组织化学法检测各组织中p16、Ki-67情况,以病理学检验结果为“金标准”,分析p16、Ki-67检测在宫颈良恶性病变中的诊断结果和效能。结果:病理学检查结果显示,90例宫颈病变患者中,高度鳞状上皮内病变25例,宫颈鳞状细胞癌31例,宫颈平滑肌瘤34例;三种宫颈病变患者的p16、Ki-67阳性表达率比较,差异均有统计学意义(P<0.05);p16联合Ki-67检测诊断宫颈良恶性病变的灵敏度、特异度、准确度均高于单一p16或Ki-67检测,差异有统计学意义(P<0.05)。结论:p16、Ki-67在宫颈恶性病变组织中高表达,p16联合Ki-67检测诊断宫颈良恶性病变的灵敏度、特异度和准确度均高于p16或Ki-67单一检测。

Objective:To observe expressions of p16 and Ki-67 in different cervical lesions and efficacies of their level detection in diagnosis of cervical benign and malignant lesions.Methods:A retrospective analysis of the clinical data of 90 patients with cervical lesions admitted to our hospital from May 2017 to May 2020 was performed.The lesion tissue was collected during the surgery and sent for examination.The expression of p16 and Ki-67 in each lesion tissue were detected by immunohistochemistry.Using the pathological test results as the“gold standard”,the diagnostic results and efficacies of p16 and Ki-67 detection in cervical benign and malignant lesions were analyzed.Results:The pathological examination results showed that among the 90 patients with cervical lesions,25 had high-grade squamous intraepithelial lesions,31 had cervical squamous cell carcinoma,and 34 had cervical leiomyomas.There were statistical differences in the p16 and Ki-67 positive expression rate among the three groups(P<0.05).The sensitivity,specificity and accuracy of p16 combined with Ki-67 detection in the diagnosis of cervical benign and malignant lesions were higher than those of single p16 detection or single Ki-67 detection,and the differences were statistically significant(P<0.05).Conclusions:p16 and Ki-67 are highly expressed in the cervical malignant lesions.The sensitivity,specificity and accuracy of p16 combined with Ki-67 detection in the diagnosis of cervical benign and malignant lesions are higher than those of single p16 detection or single Ki-67 detection.