软骨细胞分化过程中SOX9的作用

Effects of SOX9 on chondrocyte differentiation

背景:软骨细胞是软骨修复工程种子细胞的来源,软骨细胞分化与软骨内骨化密切相关,软骨内骨化是必不可少的骨骼发育过程。SOX9对软骨细胞调控起着重要作用。目的:综述SOX9在不同水平对软骨细胞分化的调控机制的最新研究进展。方法:应用计算机检索CNKI、PubMed及万方医学数据库从建库至2020年12月发表的相关文献,中文关键词为“软骨,软骨细胞,SOX9基因表达调控,转录后调控,蛋白质加工,翻译后修饰,功能伙伴”;英文关键词为“Cartilage,chondrocyte,posttranscriptional regulation,posttranslational modification,SOX9,transcriptional regulation,Gene Expression Regulation,SOX9 Transcription Factor”。最终纳入61篇符合标准的文献进行综述。结果与结论:在基因、RNA和蛋白质水平上参与SOX9调控的关键机制已经被发现,通过SOX9关键因子作用可以直接或间接促进软骨细胞成骨生长,有正向促进,也有反向抑制,以及各因素之间相互作用。SOX9是软骨细胞分化及促进软骨形成的关键因子;通过系统分析SOX9在不同水平调控的分子机制为软骨组织工程化提供理论基础,可以更快实现软骨疾病的临床治疗应用。

BACKGROUND:Chondrocytes are the source of seed cells for cartilage repair engineering.Chondrocyte differentiation is closely related to endochondral ossification,which is an essential for bone development.SOX9 plays an important role in the regulation of chondrocytes.OBJECTIVE:To review the latest research progress in the regulatory mechanism of SOX9 on chondrocyte differentiation at different levels.METHODS:A computer search was conducted for the relevant literatures published in CNKI,WanFang,and PubMed until December 2020.The key words were“cartilage,chondrocyte,regulation of SOX9 gene expression,posttranscriptional regulation,protein processing,posttranslational modification,functional partner”in Chinese and“cartilage,chondrocyte,posttranscriptional regulation,posttranslational modification,SOX9,transcriptional regulation,gene expression regulation,SOX9 transcription factor”in English,respectively.Finally,61 eligible articles were included for further review.RESULTS AND CONCLUSION:The key mechanisms involved in SOX9 regulation at the gene,RNA,and protein levels have been discovered.The SOX9 key factors can directly or indirectly promote osteogenic growth of chondrocytes,either positively or negatively,or even due to the interaction between various factors.SOX9 is a key factor in chondrocyte differentiation and chondrogenesis.By systematically analyzing the molecular mechanism of SOX9 regulation at different levels,the theoretical basis for cartilage tissue engineering can be provided,to accelerate the clinical treatment and application in cartilage diseases.