组织光透明技术在骨透明化中的生物医学成像应用与展望

Bone Transparency Imaging Using Tissue Optical Clearing Technique

近年来组织光透明技术的飞速发展,为现代骨科临床基础研究带来了新的契机。组织光透明技术主要通过多种物理、化学手段,降低组织的光散射和光吸收,使光能在组织中更好地传播,从而增加光学成像的深度和对比度。结合多种荧光标记策略,实现更深层、更高分辨的骨组织及三维空间微结构信息,为突破骨这种高散射组织及骨疾病的分子影像学研究带来新的视角与方法。对组织光透明技术的原理及机制进行介绍,并重点从骨组织光透明成像技术的应用现状、新方法及透明机制三个方面加以详述,最后对该技术应用于骨及骨疾病的分子影像学研究前景进行展望。

Recently,the rapid advancements in tissue optical clearing technology have brought new opportunities for modern orthopedic optical imaging research.Tissue optical clearing technology primarily reduces tissue light scattering and light absorption through various physical and chemical methods,allowing light energy to better spread in the tissue in order to increase the depth and contrast of optical imaging.Deeper and higher resolution bone tissue images and 3 D spatial microstructure information can be obtained when combined with various fluorescence-labeling strategies,presenting a novel perspective and method for a breakthrough in the molecular imaging study of bone tissue with high scattering and bone disease.In this study,we will discuss the principle and mechanism of tissue optical clearing technology,focusing on the current state,most recent methods,and the mechanism of bone tissue clearing imaging,and prospect the possibility of using this technique in the molecular imaging of bone.